Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23207 | 5' | -53.1 | NC_005259.1 | + | 49645 | 0.67 | 0.859869 |
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Target: 5'- gCGucGGCGaUGgcGGCCUCGauacGCGCCu -3' miRNA: 3'- aGCcuCUGCaACuuCUGGAGC----UGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 5598 | 0.67 | 0.874848 |
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Target: 5'- gCGGAGugcUGUgugaguuUGAAGACCUCauaGCCg -3' miRNA: 3'- aGCCUCu--GCA-------ACUUCUGGAGcugCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 42670 | 0.66 | 0.890383 |
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Target: 5'- gCGGcuGCuGUUGgcGAUCUCGACcCCg -3' miRNA: 3'- aGCCucUG-CAACuuCUGGAGCUGcGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 47797 | 0.66 | 0.890383 |
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Target: 5'- gCGGuccGACGgUGAgucgcgAGACCUCGcGgGCCa -3' miRNA: 3'- aGCCu--CUGCaACU------UCUGGAGC-UgCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 58162 | 0.66 | 0.897389 |
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Target: 5'- cUCGucGGCGcacGGGAUCUCGACGCa -3' miRNA: 3'- -AGCcuCUGCaacUUCUGGAGCUGCGg -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 46533 | 0.66 | 0.904136 |
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Target: 5'- gUCGGGGAuCGcc-GAGAUCUgGuCGCCg -3' miRNA: 3'- -AGCCUCU-GCaacUUCUGGAgCuGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 47900 | 0.66 | 0.904136 |
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Target: 5'- gUCGGcu-CGUUGAGcGCCUUGAuggcguCGCCg -3' miRNA: 3'- -AGCCucuGCAACUUcUGGAGCU------GCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 61076 | 0.66 | 0.910622 |
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Target: 5'- aUCGGcauAGGCGUcGucGGCCUCGGgcuuguugaGCCa -3' miRNA: 3'- -AGCC---UCUGCAaCuuCUGGAGCUg--------CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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