Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23207 | 5' | -53.1 | NC_005259.1 | + | 39554 | 0.7 | 0.689802 |
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Target: 5'- ----cGACGUUGAGccggucguccggcccGACCUCGAUGCUg -3' miRNA: 3'- agccuCUGCAACUU---------------CUGGAGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 29908 | 0.71 | 0.642398 |
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Target: 5'- -aGGGGugGcUGcccGAGACCaUCGACGCg -3' miRNA: 3'- agCCUCugCaAC---UUCUGG-AGCUGCGg -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 44969 | 0.71 | 0.631569 |
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Target: 5'- aUCGG-GAUGccGAGGGCCggugCGACuGCCg -3' miRNA: 3'- -AGCCuCUGCaaCUUCUGGa---GCUG-CGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 63233 | 0.72 | 0.599124 |
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Target: 5'- aUCGGGGuCGcUGGucAGACC-CGACGCg -3' miRNA: 3'- -AGCCUCuGCaACU--UCUGGaGCUGCGg -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 18237 | 0.72 | 0.599124 |
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Target: 5'- cCGGAGACGgagauuucgagcUgcccgccgcgccUGAAccugagacGACCUCGACGCCc -3' miRNA: 3'- aGCCUCUGC------------A------------ACUU--------CUGGAGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 32853 | 0.73 | 0.524732 |
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Target: 5'- cUCuGAGACGaacuuGACCUCGACGCg -3' miRNA: 3'- -AGcCUCUGCaacuuCUGGAGCUGCGg -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 68554 | 0.77 | 0.314743 |
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Target: 5'- aUCGGGGugGUUGAgccaaccgagguGGACCUCGuauccgGCgGCCa -3' miRNA: 3'- -AGCCUCugCAACU------------UCUGGAGC------UG-CGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 57791 | 1.14 | 0.001326 |
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Target: 5'- gUCGGAGACGUUGAAGACCUCGACGCCg -3' miRNA: 3'- -AGCCUCUGCAACUUCUGGAGCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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