Results 1 - 20 of 88 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23211 | 3' | -56.5 | NC_005259.1 | + | 39876 | 0.66 | 0.740514 |
Target: 5'- gAGCuGCGCCCacaccgaaUCGuUGUcGCCG-CCGCc -3' miRNA: 3'- -UCG-UGUGGG--------AGCuACAuCGGCuGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 37399 | 0.66 | 0.740514 |
Target: 5'- cGC-CGCCCaUGGUGUuguugacGCCGcCCGCc -3' miRNA: 3'- uCGuGUGGGaGCUACAu------CGGCuGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 46731 | 0.66 | 0.740514 |
Target: 5'- gAGCACguaggugauGCCCgCGAUGgcugcGCCGACaccuGCg -3' miRNA: 3'- -UCGUG---------UGGGaGCUACau---CGGCUGg---CG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 48626 | 0.66 | 0.740514 |
Target: 5'- cGCACugCCUCGccG-AGaCgGGCUGCc -3' miRNA: 3'- uCGUGugGGAGCuaCaUC-GgCUGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 36662 | 0.66 | 0.740514 |
Target: 5'- cGC-CGCCCUCGcc---GCCG-CCGCc -3' miRNA: 3'- uCGuGUGGGAGCuacauCGGCuGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 3977 | 0.66 | 0.740514 |
Target: 5'- gAGCGguCCCgCGAgcacgcgaUGGCgGGCCGCg -3' miRNA: 3'- -UCGUguGGGaGCUac------AUCGgCUGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 26709 | 0.66 | 0.719899 |
Target: 5'- gGGUugAuuUCCUCGAccaucgGGCCGACCuGCu -3' miRNA: 3'- -UCGugU--GGGAGCUaca---UCGGCUGG-CG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 60276 | 0.66 | 0.713646 |
Target: 5'- cAGCGCugCCgggcggccaucgucgUCGGUGcGGCCacgGACgGCg -3' miRNA: 3'- -UCGUGugGG---------------AGCUACaUCGG---CUGgCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 46426 | 0.66 | 0.709463 |
Target: 5'- uGUGCACCCgugaCGAUgGUGGCaaGCUGCu -3' miRNA: 3'- uCGUGUGGGa---GCUA-CAUCGgcUGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 23152 | 0.66 | 0.698953 |
Target: 5'- uGCGagacCACCCUCGGUGUc-CCGuucaucCCGCu -3' miRNA: 3'- uCGU----GUGGGAGCUACAucGGCu-----GGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 46340 | 0.66 | 0.697899 |
Target: 5'- cGCGCACCgCUUGuagGUucugGGCCGcgauggcACCGCc -3' miRNA: 3'- uCGUGUGG-GAGCua-CA----UCGGC-------UGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 175 | 0.66 | 0.688383 |
Target: 5'- cGGCAcCACCgaCUCGGUcaucuauggcgcGUGGCgCGACgGCa -3' miRNA: 3'- -UCGU-GUGG--GAGCUA------------CAUCG-GCUGgCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 35250 | 0.66 | 0.688383 |
Target: 5'- uGCGCACUCgCGuUGUucgcGCCcGCCGCg -3' miRNA: 3'- uCGUGUGGGaGCuACAu---CGGcUGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 43860 | 0.66 | 0.688383 |
Target: 5'- gAGCGCACCCgugaCGAcaccggcagcguUGccGCCGAcauugcCCGCg -3' miRNA: 3'- -UCGUGUGGGa---GCU------------ACauCGGCU------GGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 38814 | 0.67 | 0.677761 |
Target: 5'- cGCccGCGCCCgCGAUGaccgAGUCGAUgGCc -3' miRNA: 3'- uCG--UGUGGGaGCUACa---UCGGCUGgCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 14719 | 0.67 | 0.677761 |
Target: 5'- cAGCACGCCgcgCUCgucgccgaGAUGUAcgcGCUGGCCGg -3' miRNA: 3'- -UCGUGUGG---GAG--------CUACAU---CGGCUGGCg -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 55721 | 0.67 | 0.671367 |
Target: 5'- gAGCACcucGCCCUuguuggcaagcuuggUGGUGUcgAGaCCGugCGCa -3' miRNA: 3'- -UCGUG---UGGGA---------------GCUACA--UC-GGCugGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 3488 | 0.67 | 0.667098 |
Target: 5'- -cCACACCCUgccucacgCGAccaUGUGcGCCG-CCGCg -3' miRNA: 3'- ucGUGUGGGA--------GCU---ACAU-CGGCuGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 62585 | 0.67 | 0.667098 |
Target: 5'- cGaccuCGCCCUCGAUGagaGGCUGugugagcugacGCCGCa -3' miRNA: 3'- uCgu--GUGGGAGCUACa--UCGGC-----------UGGCG- -5' |
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23211 | 3' | -56.5 | NC_005259.1 | + | 6406 | 0.67 | 0.656403 |
Target: 5'- aGGCgguGCCCU-GAUGUGGCcCGACCu- -3' miRNA: 3'- -UCGug-UGGGAgCUACAUCG-GCUGGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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