Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23214 | 5' | -52.3 | NC_005259.1 | + | 43778 | 0.67 | 0.870767 |
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Target: 5'- cGUACuCGccgGGcAUGAgcaGCGCGGGCACCGa -3' miRNA: 3'- -CAUGcGCa--UC-UACU---CGCGCUUGUGGU- -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 3119 | 0.67 | 0.870767 |
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Target: 5'- uUGCGCGgcaccGUGGGCaGCGGacgGCACCGc -3' miRNA: 3'- cAUGCGCauc--UACUCG-CGCU---UGUGGU- -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 6204 | 0.66 | 0.878623 |
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Target: 5'- -aGCGUGagcgGGGUGAGCcgccGCGccGACACCGu -3' miRNA: 3'- caUGCGCa---UCUACUCG----CGC--UUGUGGU- -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 57340 | 0.66 | 0.885472 |
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Target: 5'- uGUGCGCuGUccucucaAGAUGAGagaaCGGGCACCGu -3' miRNA: 3'- -CAUGCG-CA-------UCUACUCgc--GCUUGUGGU- -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 57440 | 0.66 | 0.893553 |
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Target: 5'- uUACGCGgcgAGGUuGGUGCGAcGCugCGg -3' miRNA: 3'- cAUGCGCa--UCUAcUCGCGCU-UGugGU- -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 16311 | 0.66 | 0.907406 |
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Target: 5'- -cACGUGU-GAUGuGgGCGGAUACUg -3' miRNA: 3'- caUGCGCAuCUACuCgCGCUUGUGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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