Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23217 | 3' | -57.4 | NC_005259.1 | + | 10476 | 0.66 | 0.61767 |
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Target: 5'- aGCGGGACgaucuuggggucGUGGUuguUGGGGUUGuCCGGCAc -3' miRNA: 3'- -UGUCCUG------------UAUCG---GCUCCGAC-GGCUGU- -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 6210 | 0.66 | 0.628466 |
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Target: 5'- aGCGGGGug-AGCCGccGC-GCCGACAc -3' miRNA: 3'- -UGUCCUguaUCGGCucCGaCGGCUGU- -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 6084 | 0.68 | 0.515847 |
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Target: 5'- aACAGGACGcccucaacgccgagaUcGCCGAGGCcgagGCCGcCGc -3' miRNA: 3'- -UGUCCUGU---------------AuCGGCUCCGa---CGGCuGU- -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 5956 | 0.67 | 0.571497 |
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Target: 5'- aGCGGGACuucugcgGGCCGAccaccaacuacacgGGCaGCCGAgAa -3' miRNA: 3'- -UGUCCUGua-----UCGGCU--------------CCGaCGGCUgU- -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 2160 | 0.67 | 0.585392 |
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Target: 5'- cCAGcGGCGUcgAGCCGccGGUUGCCGAg- -3' miRNA: 3'- uGUC-CUGUA--UCGGCu-CCGACGGCUgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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