Results 1 - 20 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23219 | 3' | -54.7 | NC_005259.1 | + | 8283 | 0.65 | 0.832225 |
Target: 5'- --uACCGGGugcGCGGGCAcccgcacggaaacGCCGCCUuguCGGa -3' miRNA: 3'- auuUGGCUC---UGUCUGU-------------UGGCGGG---GCC- -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 47941 | 0.66 | 0.824203 |
Target: 5'- gAAGCCGAuGACGgcggugccGAUGGCCGCCgCGc -3' miRNA: 3'- aUUUGGCU-CUGU--------CUGUUGGCGGgGCc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 1910 | 0.66 | 0.824203 |
Target: 5'- --uGCCGAGGaAGcGC-GCCGCCCCa- -3' miRNA: 3'- auuUGGCUCUgUC-UGuUGGCGGGGcc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 18270 | 0.66 | 0.823301 |
Target: 5'- cUGAACCuGAGACGaccucgacgcccGACgAGCCGCcguucugCCCGGu -3' miRNA: 3'- -AUUUGG-CUCUGU------------CUG-UUGGCG-------GGGCC- -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 63658 | 0.66 | 0.815103 |
Target: 5'- -uGGCCGGGACgccGGACAccucgggcaccGgCGCgCCGGg -3' miRNA: 3'- auUUGGCUCUG---UCUGU-----------UgGCGgGGCC- -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 46909 | 0.66 | 0.805817 |
Target: 5'- --cGCCGAG-CAGuccGCGugCGCCgCCGa -3' miRNA: 3'- auuUGGCUCuGUC---UGUugGCGG-GGCc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 4221 | 0.66 | 0.796355 |
Target: 5'- gAGAUCGAG-CAGGCcaAGCagGCCCCGc -3' miRNA: 3'- aUUUGGCUCuGUCUG--UUGg-CGGGGCc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 21386 | 0.66 | 0.796355 |
Target: 5'- --cGCCGAGGCGGuGCAACgCGCaugggacgCCCGu -3' miRNA: 3'- auuUGGCUCUGUC-UGUUG-GCG--------GGGCc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 29187 | 0.66 | 0.796355 |
Target: 5'- cGAACCcguGGGGCGGAUggUuCGCCCCc- -3' miRNA: 3'- aUUUGG---CUCUGUCUGuuG-GCGGGGcc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 47491 | 0.66 | 0.7954 |
Target: 5'- --cACCGAGGCGcGGCAggaacacGCUGCCCUc- -3' miRNA: 3'- auuUGGCUCUGU-CUGU-------UGGCGGGGcc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 48636 | 0.66 | 0.786727 |
Target: 5'- --cGCCGAGACGGGCugccaGAUCGUCgCCa- -3' miRNA: 3'- auuUGGCUCUGUCUG-----UUGGCGG-GGcc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 44867 | 0.67 | 0.776943 |
Target: 5'- cAGACCGGcGGCgAGAucCGACgCGCCCCa- -3' miRNA: 3'- aUUUGGCU-CUG-UCU--GUUG-GCGGGGcc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 49991 | 0.67 | 0.776943 |
Target: 5'- cGGAUCGcGuGCGGACGGCUGCCCuCGu -3' miRNA: 3'- aUUUGGCuC-UGUCUGUUGGCGGG-GCc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 14245 | 0.67 | 0.776943 |
Target: 5'- cGGGCCG-GGCAacGACGACCGugucgucaCCCCGu -3' miRNA: 3'- aUUUGGCuCUGU--CUGUUGGC--------GGGGCc -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 4511 | 0.67 | 0.776943 |
Target: 5'- --cGCCGGGAguGGCGcuGCgCGgcaCCCCGGu -3' miRNA: 3'- auuUGGCUCUguCUGU--UG-GC---GGGGCC- -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 778 | 0.67 | 0.776943 |
Target: 5'- --cGCCGAGGCG----GCUGCCCuCGGu -3' miRNA: 3'- auuUGGCUCUGUcuguUGGCGGG-GCC- -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 3127 | 0.67 | 0.767013 |
Target: 5'- --cACCGuGggcaGCGGACGgcACCGCCCgUGGg -3' miRNA: 3'- auuUGGCuC----UGUCUGU--UGGCGGG-GCC- -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 26327 | 0.67 | 0.756949 |
Target: 5'- ---cUCGGGgucgcACAGAUAGCaCGCCCuCGGg -3' miRNA: 3'- auuuGGCUC-----UGUCUGUUG-GCGGG-GCC- -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 14175 | 0.67 | 0.753905 |
Target: 5'- --cGCCGGGcgGguGGCAACCGCUcacccccgagcaggCCGGg -3' miRNA: 3'- auuUGGCUC--UguCUGUUGGCGG--------------GGCC- -5' |
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23219 | 3' | -54.7 | NC_005259.1 | + | 12559 | 0.67 | 0.746761 |
Target: 5'- --uGCCGGGGuggcccgcCAGAUccauGCCGCCCCa- -3' miRNA: 3'- auuUGGCUCU--------GUCUGu---UGGCGGGGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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