Results 1 - 20 of 52 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23223 | 5' | -61.5 | NC_005259.1 | + | 60270 | 0.66 | 0.495644 |
Target: 5'- gGCCaccaGCGCUGcCGgGCggccaUCGUCGUCGg -3' miRNA: 3'- gCGG----CGUGAC-GCgCGac---AGCGGCAGC- -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 8472 | 0.66 | 0.495644 |
Target: 5'- aGCUGCACgagcacCGCGCUGUCGgCa--- -3' miRNA: 3'- gCGGCGUGac----GCGCGACAGCgGcagc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 34715 | 0.66 | 0.495644 |
Target: 5'- uGCCGCGCgaGCGC-CUcGUCGgggaaaCCGUUGg -3' miRNA: 3'- gCGGCGUGa-CGCGcGA-CAGC------GGCAGC- -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 36764 | 0.66 | 0.485974 |
Target: 5'- gCGUgaCGUuagGCGCGUUGcCGCCGUCc -3' miRNA: 3'- -GCG--GCGugaCGCGCGACaGCGGCAGc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 5682 | 0.66 | 0.485974 |
Target: 5'- uCGCCccGCACUGCcUGCUGUgcucgUGCCGcCa -3' miRNA: 3'- -GCGG--CGUGACGcGCGACA-----GCGGCaGc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 8820 | 0.66 | 0.485974 |
Target: 5'- gCGCCGgGCcggugaguUGaCGCGCUG-CGCCGa-- -3' miRNA: 3'- -GCGGCgUG--------AC-GCGCGACaGCGGCagc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 26947 | 0.66 | 0.485974 |
Target: 5'- aCGCCGCuGCUGgaaCGUGCgcagCGCCGUg- -3' miRNA: 3'- -GCGGCG-UGAC---GCGCGaca-GCGGCAgc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 17314 | 0.66 | 0.484051 |
Target: 5'- aCGCCGaGCgguaccccgaggGUGUGCcggucaacGUCGCCGUCGa -3' miRNA: 3'- -GCGGCgUGa-----------CGCGCGa-------CAGCGGCAGC- -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 13438 | 0.66 | 0.476395 |
Target: 5'- gGCCGCGCUagGCGUGaCcGUCGgCG-CGg -3' miRNA: 3'- gCGGCGUGA--CGCGC-GaCAGCgGCaGC- -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 58838 | 0.66 | 0.470693 |
Target: 5'- uCGCCGCcCUcggucucacccucggGCuCGCcGUCGUCGUCGu -3' miRNA: 3'- -GCGGCGuGA---------------CGcGCGaCAGCGGCAGC- -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 37221 | 0.66 | 0.46691 |
Target: 5'- cCGCCGC-CgGgGCGCUGgcccgUGCCGg-- -3' miRNA: 3'- -GCGGCGuGaCgCGCGACa----GCGGCagc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 6447 | 0.66 | 0.46691 |
Target: 5'- cCGUCGCGCUGaucgGCaGC-GUCGCCGg-- -3' miRNA: 3'- -GCGGCGUGACg---CG-CGaCAGCGGCagc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 38189 | 0.66 | 0.46691 |
Target: 5'- uCGCUGUACU-CGUagaccUUGUCGCCGUUGa -3' miRNA: 3'- -GCGGCGUGAcGCGc----GACAGCGGCAGC- -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 26024 | 0.66 | 0.46691 |
Target: 5'- gGCCGCAa-GCGgGUUGgccagcagCGCCGcCGg -3' miRNA: 3'- gCGGCGUgaCGCgCGACa-------GCGGCaGC- -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 5421 | 0.66 | 0.46691 |
Target: 5'- gGCCGCcacgucgagguCUGCGCccGCUGcCGCCGa-- -3' miRNA: 3'- gCGGCGu----------GACGCG--CGACaGCGGCagc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 39316 | 0.66 | 0.457525 |
Target: 5'- aCGcCCGCugUcGCGcCGCUGgUGaCCGUCa -3' miRNA: 3'- -GC-GGCGugA-CGC-GCGACaGC-GGCAGc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 50018 | 0.66 | 0.457525 |
Target: 5'- uGCacggGCGCUGCGUaauccGCUGUgGCC-UCGa -3' miRNA: 3'- gCGg---CGUGACGCG-----CGACAgCGGcAGC- -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 50686 | 0.66 | 0.448241 |
Target: 5'- uGCCG-ACgagGCGCUGcUCGUCGUCGc -3' miRNA: 3'- gCGGCgUGacgCGCGAC-AGCGGCAGC- -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 14706 | 0.66 | 0.439062 |
Target: 5'- cCGCCGCgACgaacagcacGcCGCGCUcGUCGCCGa-- -3' miRNA: 3'- -GCGGCG-UGa--------C-GCGCGA-CAGCGGCagc -5' |
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23223 | 5' | -61.5 | NC_005259.1 | + | 61473 | 0.66 | 0.439062 |
Target: 5'- uGCCGauugacCUGCaCGCUGUCGCgacCGUUGa -3' miRNA: 3'- gCGGCgu----GACGcGCGACAGCG---GCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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