Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23231 | 5' | -61.6 | NC_005259.1 | + | 45504 | 0.66 | 0.461074 |
Target: 5'- uGGUCGCuGuGGCCacCGCGccgguguuGGCCGCCGu -3' miRNA: 3'- cUCAGCG-CuCUGGa-GCGC--------CCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 37522 | 0.67 | 0.398179 |
Target: 5'- ---cCGaCGAGAagaUCGuCGGGCCGCCGu -3' miRNA: 3'- cucaGC-GCUCUgg-AGC-GCCCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 17819 | 0.67 | 0.406826 |
Target: 5'- uGAGcUCaGCGAGGCCaUCGaCGGGUgGgCCGa -3' miRNA: 3'- -CUC-AG-CGCUCUGG-AGC-GCCCGgU-GGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 55876 | 0.67 | 0.415589 |
Target: 5'- cGAGcgccUCGCcccGACCUCGgGGGUCgcGCCGg -3' miRNA: 3'- -CUC----AGCGcu-CUGGAGCgCCCGG--UGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 58288 | 0.67 | 0.415589 |
Target: 5'- aGGuUCGCGAGGCCa-GCGGauaCCGCCa -3' miRNA: 3'- cUC-AGCGCUCUGGagCGCCc--GGUGGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 40063 | 0.67 | 0.423574 |
Target: 5'- cGGUCGCGAuGACC-CaUGGGCCgacgcgcACCGa -3' miRNA: 3'- cUCAGCGCU-CUGGaGcGCCCGG-------UGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 31280 | 0.67 | 0.433457 |
Target: 5'- cGGUgGCGAGAUCUUGCccGGUCugCGc -3' miRNA: 3'- cUCAgCGCUCUGGAGCGc-CCGGugGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 41311 | 0.66 | 0.442557 |
Target: 5'- uGAG-CGCGAggucGACCUCGaaaaGGGCgguGCCGu -3' miRNA: 3'- -CUCaGCGCU----CUGGAGCg---CCCGg--UGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 30133 | 0.66 | 0.451763 |
Target: 5'- cGGcUGCGAuACCUCGggcagcucgacCGGGCCGCCu -3' miRNA: 3'- cUCaGCGCUcUGGAGC-----------GCCCGGUGGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 27548 | 0.66 | 0.451763 |
Target: 5'- ----gGCGGGAUgUUGC-GGCCACCGa -3' miRNA: 3'- cucagCGCUCUGgAGCGcCCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 14565 | 0.67 | 0.398179 |
Target: 5'- cGAG-CG-GGGACCggcgaacaccaUCGCGcuGGCCACCGa -3' miRNA: 3'- -CUCaGCgCUCUGG-----------AGCGC--CCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 62657 | 0.66 | 0.461074 |
Target: 5'- -cGUUGuCGAGACCgaGCGaGGCCACg- -3' miRNA: 3'- cuCAGC-GCUCUGGagCGC-CCGGUGgc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 23511 | 0.66 | 0.461074 |
Target: 5'- cGAGcucagCGaCGAGAUCUCGaCGGugaaaaucaGCCGCCGc -3' miRNA: 3'- -CUCa----GC-GCUCUGGAGC-GCC---------CGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 6103 | 0.66 | 0.466709 |
Target: 5'- cGAGaUCGcCGAGGCCgaggccgccgcuaCGCGGGCaCugCa -3' miRNA: 3'- -CUC-AGC-GCUCUGGa------------GCGCCCG-GugGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 45397 | 0.66 | 0.470485 |
Target: 5'- gGGGUCGCc--GCCgagCGCGcugauGGCCGCCGc -3' miRNA: 3'- -CUCAGCGcucUGGa--GCGC-----CCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 48476 | 0.66 | 0.470485 |
Target: 5'- cGGGUCGCGucGGCCUguuccucgacgCGCuugaGGGCCGCg- -3' miRNA: 3'- -CUCAGCGCu-CUGGA-----------GCG----CCCGGUGgc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 23346 | 0.66 | 0.470485 |
Target: 5'- cGGG-CuCGAGGCUgccUGCGaGGCCGCCGa -3' miRNA: 3'- -CUCaGcGCUCUGGa--GCGC-CCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 56361 | 0.66 | 0.470485 |
Target: 5'- cGGGcaCGCGGuacaGCCgUCGCGGGCCGCg- -3' miRNA: 3'- -CUCa-GCGCUc---UGG-AGCGCCCGGUGgc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 12011 | 0.66 | 0.479038 |
Target: 5'- aGAGUCG-GuGGCCUuguacggUGCGGGCggcaACCGg -3' miRNA: 3'- -CUCAGCgCuCUGGA-------GCGCCCGg---UGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 45548 | 0.66 | 0.479993 |
Target: 5'- ----aGCGGGuugCUCGcCGGGCCACCc -3' miRNA: 3'- cucagCGCUCug-GAGC-GCCCGGUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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