Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23231 | 5' | -61.6 | NC_005259.1 | + | 5956 | 0.72 | 0.206423 |
Target: 5'- ----aGCGGGACUUCuGCGGGCCgACCa -3' miRNA: 3'- cucagCGCUCUGGAG-CGCCCGG-UGGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 6103 | 0.66 | 0.466709 |
Target: 5'- cGAGaUCGcCGAGGCCgaggccgccgcuaCGCGGGCaCugCa -3' miRNA: 3'- -CUC-AGC-GCUCUGGa------------GCGCCCG-GugGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 7664 | 0.67 | 0.398179 |
Target: 5'- -cGUCGUGAGcgccccGCCUCGUGgGGUgGCCc -3' miRNA: 3'- cuCAGCGCUC------UGGAGCGC-CCGgUGGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 8387 | 0.7 | 0.263773 |
Target: 5'- --aUCgGCGAGAgCCUCGgcgagcCGGGCCACCu -3' miRNA: 3'- cucAG-CGCUCU-GGAGC------GCCCGGUGGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 9516 | 0.7 | 0.283337 |
Target: 5'- -cGUCGCGcgcgucGACCgcCGcCGGGUCACCGg -3' miRNA: 3'- cuCAGCGCu-----CUGGa-GC-GCCCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 12011 | 0.66 | 0.479038 |
Target: 5'- aGAGUCG-GuGGCCUuguacggUGCGGGCggcaACCGg -3' miRNA: 3'- -CUCAGCgCuCUGGA-------GCGCCCGg---UGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 13213 | 0.68 | 0.333401 |
Target: 5'- cGGGgccaGCGguGGGCCUCGaCcGGCCACCGu -3' miRNA: 3'- -CUCag--CGC--UCUGGAGC-GcCCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 14565 | 0.67 | 0.398179 |
Target: 5'- cGAG-CG-GGGACCggcgaacaccaUCGCGcuGGCCACCGa -3' miRNA: 3'- -CUCaGCgCUCUGG-----------AGCGC--CCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 17819 | 0.67 | 0.406826 |
Target: 5'- uGAGcUCaGCGAGGCCaUCGaCGGGUgGgCCGa -3' miRNA: 3'- -CUC-AG-CGCUCUGG-AGC-GCCCGgU-GGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 18690 | 0.68 | 0.35676 |
Target: 5'- aGGGUCugcuugagcacgGCGGucuGACCUCGaucauCGGGCCGCCc -3' miRNA: 3'- -CUCAG------------CGCU---CUGGAGC-----GCCCGGUGGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 19236 | 0.68 | 0.381245 |
Target: 5'- cGAGUCgGCGAugaucgauGGCCgccUGcCGGGCCGCCc -3' miRNA: 3'- -CUCAG-CGCU--------CUGGa--GC-GCCCGGUGGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 22925 | 0.68 | 0.35676 |
Target: 5'- --cUCGUGcucGACCUCGcCGGGUCAUCGc -3' miRNA: 3'- cucAGCGCu--CUGGAGC-GCCCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 23346 | 0.66 | 0.470485 |
Target: 5'- cGGG-CuCGAGGCUgccUGCGaGGCCGCCGa -3' miRNA: 3'- -CUCaGcGCUCUGGa--GCGC-CCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 23511 | 0.66 | 0.461074 |
Target: 5'- cGAGcucagCGaCGAGAUCUCGaCGGugaaaaucaGCCGCCGc -3' miRNA: 3'- -CUCa----GC-GCUCUGGAGC-GCC---------CGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 27548 | 0.66 | 0.451763 |
Target: 5'- ----gGCGGGAUgUUGC-GGCCACCGa -3' miRNA: 3'- cucagCGCUCUGgAGCGcCCGGUGGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 27904 | 0.72 | 0.19145 |
Target: 5'- gGGGUCGcCGAGACCgCGCGGGUggUAgCGg -3' miRNA: 3'- -CUCAGC-GCUCUGGaGCGCCCG--GUgGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 29909 | 0.68 | 0.348848 |
Target: 5'- gGGGUgGCugcccGAGACCaucgaCGCGuGGCCGCCa -3' miRNA: 3'- -CUCAgCG-----CUCUGGa----GCGC-CCGGUGGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 30133 | 0.66 | 0.451763 |
Target: 5'- cGGcUGCGAuACCUCGggcagcucgacCGGGCCGCCu -3' miRNA: 3'- cUCaGCGCUcUGGAGC-----------GCCCGGUGGc -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 31280 | 0.67 | 0.433457 |
Target: 5'- cGGUgGCGAGAUCUUGCccGGUCugCGc -3' miRNA: 3'- cUCAgCGCUCUGGAGCGc-CCGGugGC- -5' |
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23231 | 5' | -61.6 | NC_005259.1 | + | 33588 | 0.71 | 0.226273 |
Target: 5'- -cGUCGCGGGccACCgaggCGCGgucgagcugcaaccGGCCACCGa -3' miRNA: 3'- cuCAGCGCUC--UGGa---GCGC--------------CCGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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