Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23232 | 3' | -56.1 | NC_005259.1 | + | 24553 | 0.66 | 0.769097 |
Target: 5'- cCAGaUCGgCGGGGUGuacUCGUGggCACCg -3' miRNA: 3'- aGUC-GGUgGCUCCACu--AGCGCa-GUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 45734 | 0.66 | 0.749169 |
Target: 5'- gCA-CCACCGAGGc---CGCG-CACCg -3' miRNA: 3'- aGUcGGUGGCUCCacuaGCGCaGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 11015 | 0.66 | 0.728793 |
Target: 5'- cCGGaCgCGCCGAGGcagGAguUCGCcgggugGUCACCg -3' miRNA: 3'- aGUC-G-GUGGCUCCa--CU--AGCG------CAGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 6440 | 0.66 | 0.759194 |
Target: 5'- gUCGG-CACCGucGcgcUGAUCggcaGCGUCGCCg -3' miRNA: 3'- -AGUCgGUGGCucC---ACUAG----CGCAGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 60929 | 0.66 | 0.739032 |
Target: 5'- -gAGCUcgGCCuuGGUGAUCGUcugCACCa -3' miRNA: 3'- agUCGG--UGGcuCCACUAGCGca-GUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 20773 | 0.67 | 0.708052 |
Target: 5'- cCGcCCACCG-GGUGG-CGCGUCgacagcaagaGCCa -3' miRNA: 3'- aGUcGGUGGCuCCACUaGCGCAG----------UGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 66931 | 0.67 | 0.708052 |
Target: 5'- --uGCUcgACCGGGGUGAgCGgGaCACCg -3' miRNA: 3'- aguCGG--UGGCUCCACUaGCgCaGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 25926 | 0.67 | 0.718463 |
Target: 5'- --cGCCGCCGGuccGGUGAgCGCa-CGCCg -3' miRNA: 3'- aguCGGUGGCU---CCACUaGCGcaGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 52403 | 0.67 | 0.697572 |
Target: 5'- -gGGCCAUCGAGGUGugggCGC-UCgACUc -3' miRNA: 3'- agUCGGUGGCUCCACua--GCGcAG-UGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 33131 | 0.67 | 0.687031 |
Target: 5'- gCAGCCGCCGcGcGUGAUCuugcCGUagACCg -3' miRNA: 3'- aGUCGGUGGCuC-CACUAGc---GCAg-UGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 53725 | 0.67 | 0.67644 |
Target: 5'- --cGCCGCUGAuGGUGA---UGUCGCCg -3' miRNA: 3'- aguCGGUGGCU-CCACUagcGCAGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 65562 | 0.67 | 0.685974 |
Target: 5'- aUCGGCCcauagacACCGAGG-GAcggGCGUCaaACCg -3' miRNA: 3'- -AGUCGG-------UGGCUCCaCUag-CGCAG--UGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 28229 | 0.67 | 0.697572 |
Target: 5'- --cGCCACCGucggcggcgGGGUGGUCGa-UCACg -3' miRNA: 3'- aguCGGUGGC---------UCCACUAGCgcAGUGg -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 46589 | 0.67 | 0.718463 |
Target: 5'- --cGCCGcCCGAGGcGAgcagCGCGUCGu- -3' miRNA: 3'- aguCGGU-GGCUCCaCUa---GCGCAGUgg -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 5553 | 0.67 | 0.718463 |
Target: 5'- -aGGCCGCgUGGGGUGGUgacgGCGagACCg -3' miRNA: 3'- agUCGGUG-GCUCCACUAg---CGCagUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 51488 | 0.68 | 0.612404 |
Target: 5'- aCA-CCGCCGGGGauaGGUCGaCGcCGCCg -3' miRNA: 3'- aGUcGGUGGCUCCa--CUAGC-GCaGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 903 | 0.68 | 0.644471 |
Target: 5'- ----aCACCGAGGUGGUgcccgGCGaUCACCu -3' miRNA: 3'- agucgGUGGCUCCACUAg----CGC-AGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 39089 | 0.68 | 0.63378 |
Target: 5'- aCAGUCcguugagcggGuuGAGGUGcugcaAUCGCGUCACg -3' miRNA: 3'- aGUCGG----------UggCUCCAC-----UAGCGCAGUGg -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 45529 | 0.68 | 0.63378 |
Target: 5'- gUUGGCCGCCGuGGcugcgagcgGGUUGC-UCGCCg -3' miRNA: 3'- -AGUCGGUGGCuCCa--------CUAGCGcAGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 23366 | 0.68 | 0.63378 |
Target: 5'- -aGGCCGCCGAGGcaugGAUCGUcaaCAgCg -3' miRNA: 3'- agUCGGUGGCUCCa---CUAGCGca-GUgG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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