Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23232 | 3' | -56.1 | NC_005259.1 | + | 28015 | 0.73 | 0.376119 |
Target: 5'- -gGGUCGCCGGGGuUGcgCGCGUUggccaGCCa -3' miRNA: 3'- agUCGGUGGCUCC-ACuaGCGCAG-----UGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 24553 | 0.66 | 0.769097 |
Target: 5'- cCAGaUCGgCGGGGUGuacUCGUGggCACCg -3' miRNA: 3'- aGUC-GGUgGCUCCACu--AGCGCa-GUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 4476 | 0.71 | 0.477717 |
Target: 5'- -aGGCCAUCGGcgguggaccauGGUGAUCGCcaccgCGCCg -3' miRNA: 3'- agUCGGUGGCU-----------CCACUAGCGca---GUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 21616 | 0.71 | 0.467908 |
Target: 5'- aCA-CCACCGAGGcUGuGUCGCGcggaguguUCGCCg -3' miRNA: 3'- aGUcGGUGGCUCC-AC-UAGCGC--------AGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 50795 | 0.71 | 0.458202 |
Target: 5'- gUCGGaCCAUCGAGGUucuuguucGGUCaGCGcgCACCa -3' miRNA: 3'- -AGUC-GGUGGCUCCA--------CUAG-CGCa-GUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 47794 | 0.71 | 0.458202 |
Target: 5'- gCAGCgGuCCGAcGGUGAgUCGCGagACCu -3' miRNA: 3'- aGUCGgU-GGCU-CCACU-AGCGCagUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 47900 | 0.71 | 0.448606 |
Target: 5'- gUCGGCuCGuuGAGcGccuUGAUgGCGUCGCCg -3' miRNA: 3'- -AGUCG-GUggCUC-C---ACUAgCGCAGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 36135 | 0.71 | 0.439122 |
Target: 5'- -gGGCCGCCGGGcUG-UC-CGUCGCCa -3' miRNA: 3'- agUCGGUGGCUCcACuAGcGCAGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 16176 | 0.71 | 0.439122 |
Target: 5'- -uGGCCGCCGAGGUccaagaGAUCaUGUcCACCc -3' miRNA: 3'- agUCGGUGGCUCCA------CUAGcGCA-GUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 36437 | 0.72 | 0.420503 |
Target: 5'- gCAGCguCGCCGAGGUcGAcCGCGUCGa- -3' miRNA: 3'- aGUCG--GUGGCUCCA-CUaGCGCAGUgg -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 40886 | 0.73 | 0.376119 |
Target: 5'- cCGGCCACCGuGGcggGGUCggcaGCGUC-CCa -3' miRNA: 3'- aGUCGGUGGCuCCa--CUAG----CGCAGuGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 47468 | 1.13 | 0.000662 |
Target: 5'- gUCAGCCACCGAGGUGAUCGCGUCACCg -3' miRNA: 3'- -AGUCGGUGGCUCCACUAGCGCAGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 47275 | 0.73 | 0.359273 |
Target: 5'- cCAGCgCACCGAGcaGAcCGCcGUCGCCg -3' miRNA: 3'- aGUCG-GUGGCUCcaCUaGCG-CAGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 16697 | 0.73 | 0.342159 |
Target: 5'- -uGGgCACCGAGGUGAccgccgagcaggUCGCGcucugccUCGCCa -3' miRNA: 3'- agUCgGUGGCUCCACU------------AGCGC-------AGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 53152 | 0.73 | 0.335007 |
Target: 5'- aUCAGCCgcuacGCCGAGGccGAUCuCGcCACCg -3' miRNA: 3'- -AGUCGG-----UGGCUCCa-CUAGcGCaGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 36945 | 0.73 | 0.335007 |
Target: 5'- gCuGUCACCGGGGUGccCGCGagACCg -3' miRNA: 3'- aGuCGGUGGCUCCACuaGCGCagUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 19717 | 0.77 | 0.193149 |
Target: 5'- gCuGCCGCCGAGGUGAUCGaCGgggacuaguugacUUACCc -3' miRNA: 3'- aGuCGGUGGCUCCACUAGC-GC-------------AGUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 46321 | 0.78 | 0.183792 |
Target: 5'- gCGGCC-CCGAGG--GUCGCGUCGCg -3' miRNA: 3'- aGUCGGuGGCUCCacUAGCGCAGUGg -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 58447 | 0.78 | 0.179029 |
Target: 5'- aUCGGUgGCCuuGGUGAUCGCGgcCACCa -3' miRNA: 3'- -AGUCGgUGGcuCCACUAGCGCa-GUGG- -5' |
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23232 | 3' | -56.1 | NC_005259.1 | + | 63179 | 0.81 | 0.104438 |
Target: 5'- --cGCCGCCGAGGUGAUCGCaccccggucGUgCGCCu -3' miRNA: 3'- aguCGGUGGCUCCACUAGCG---------CA-GUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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