Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23232 | 5' | -54.7 | NC_005259.1 | + | 63693 | 0.66 | 0.76843 |
Target: 5'- cCGGGGAUcuGCGcCUGCCCagcggggaUCgg-GGCCu -3' miRNA: 3'- cGUCCUUG--UGC-GACGGG--------AGaaaCCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 58813 | 0.66 | 0.777425 |
Target: 5'- gGCGGGcuCGCGCUGCucggcacCCUCgcc-GCCc -3' miRNA: 3'- -CGUCCuuGUGCGACG-------GGAGaaacCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 32177 | 0.66 | 0.788255 |
Target: 5'- cGCGuGAGCuCGCUGCgcgCCUCggucugcgacUUGGCCu -3' miRNA: 3'- -CGUcCUUGuGCGACG---GGAGa---------AACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 59858 | 0.66 | 0.788255 |
Target: 5'- -gAGGGAuCAUGCUgGCUCguugUGGCCg -3' miRNA: 3'- cgUCCUU-GUGCGA-CGGGagaaACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 60177 | 0.66 | 0.794082 |
Target: 5'- gGCGGcGAACGC-CUGCUCgaccgaccgGGCCa -3' miRNA: 3'- -CGUC-CUUGUGcGACGGGagaaa----CCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 26328 | 0.66 | 0.807441 |
Target: 5'- uCGGGGucGCACagauaGCacGCCCUCg--GGCCg -3' miRNA: 3'- cGUCCU--UGUG-----CGa-CGGGAGaaaCCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 47635 | 0.66 | 0.792146 |
Target: 5'- cGCcGaGGGCA-GCgagGCCCUCUUugaacgugcuguugaUGGCCu -3' miRNA: 3'- -CGuC-CUUGUgCGa--CGGGAGAA---------------ACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 36653 | 0.66 | 0.807441 |
Target: 5'- uCAGcGAGC-CGCcGCCCUCgcc-GCCg -3' miRNA: 3'- cGUC-CUUGuGCGaCGGGAGaaacCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 61657 | 0.67 | 0.737683 |
Target: 5'- uCAGcucAACGCGCUGCCCaUCggcGGCa -3' miRNA: 3'- cGUCc--UUGUGCGACGGG-AGaaaCCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 49987 | 0.67 | 0.748052 |
Target: 5'- cGCAcGGAuCGCgugcggacgGCUGCCCUCgugcacgGGCg -3' miRNA: 3'- -CGU-CCUuGUG---------CGACGGGAGaaa----CCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 44463 | 0.67 | 0.716643 |
Target: 5'- cCGGGGAUGcCGCcGCCCUggccgGGCCa -3' miRNA: 3'- cGUCCUUGU-GCGaCGGGAgaaa-CCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 20527 | 0.68 | 0.6519 |
Target: 5'- aGCAcGggUGCGCUGCCCgcagcgUGGUg -3' miRNA: 3'- -CGUcCuuGUGCGACGGGagaa--ACCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 42039 | 0.68 | 0.662797 |
Target: 5'- uCGGGucGACuGCGCUGUCCUCggggUGGgCg -3' miRNA: 3'- cGUCC--UUG-UGCGACGGGAGaa--ACCgG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 67441 | 0.68 | 0.673665 |
Target: 5'- cGCuuGAGCGCGUcggggaUGCCCUUUUcGGCg -3' miRNA: 3'- -CGucCUUGUGCG------ACGGGAGAAaCCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 28157 | 0.68 | 0.680168 |
Target: 5'- --cGGccucGACGCGCUGCCCUUgacguaguccggUGGCg -3' miRNA: 3'- cguCC----UUGUGCGACGGGAGaa----------ACCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 26790 | 0.68 | 0.683413 |
Target: 5'- --cGGAACAccagcgcCGCcGCCCgg-UUGGCCg -3' miRNA: 3'- cguCCUUGU-------GCGaCGGGagaAACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 32528 | 0.68 | 0.684494 |
Target: 5'- gGCGGGcGGCAgGCuUGaCCUUgcugUUGGCCg -3' miRNA: 3'- -CGUCC-UUGUgCG-ACgGGAGa---AACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 14865 | 0.68 | 0.695274 |
Target: 5'- cGCgAGGuuCGCGCUGCCCgagcc-GCCg -3' miRNA: 3'- -CG-UCCuuGUGCGACGGGagaaacCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 50633 | 0.69 | 0.596258 |
Target: 5'- aGCGGuccaugcGAuUACGCcGCCCUCgg-GGCCa -3' miRNA: 3'- -CGUC-------CUuGUGCGaCGGGAGaaaCCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 38583 | 0.7 | 0.575664 |
Target: 5'- uCAGGAugAUGCUGCCCgauccGGUg -3' miRNA: 3'- cGUCCUugUGCGACGGGagaaaCCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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