Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23232 | 5' | -54.7 | NC_005259.1 | + | 47502 | 1.14 | 0.000594 |
Target: 5'- gGCAGGAACACGCUGCCCUCUUUGGCCa -3' miRNA: 3'- -CGUCCUUGUGCGACGGGAGAAACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 44295 | 0.74 | 0.363267 |
Target: 5'- aGCGcGAGCGgGUUGCCCUCUUUGacgagcguguugagcGCCg -3' miRNA: 3'- -CGUcCUUGUgCGACGGGAGAAAC---------------CGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 35059 | 0.73 | 0.385922 |
Target: 5'- cGCcGG-ACACGCcGCCCUUgcugacggUGGCCu -3' miRNA: 3'- -CGuCCuUGUGCGaCGGGAGaa------ACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 37502 | 0.72 | 0.461182 |
Target: 5'- gGCGGuGAGCACgauGCUGCCCgacgagaagaUCgucgGGCCg -3' miRNA: 3'- -CGUC-CUUGUG---CGACGGG----------AGaaa-CCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 28360 | 0.72 | 0.47113 |
Target: 5'- --cGGuGCugGCUGCCCcacCUcgGGCCg -3' miRNA: 3'- cguCCuUGugCGACGGGa--GAaaCCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 21445 | 0.71 | 0.491346 |
Target: 5'- uGCAcauGGAGaACGCUGCCCgugaggCUUUGaaGCCg -3' miRNA: 3'- -CGU---CCUUgUGCGACGGGa-----GAAAC--CGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 776 | 0.71 | 0.491346 |
Target: 5'- cGCGccGAgGCgGCUGCCCUCgguugggUGGCCa -3' miRNA: 3'- -CGUccUUgUG-CGACGGGAGaa-----ACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 68704 | 0.71 | 0.522391 |
Target: 5'- gGCGGGcAGCGCGUcggUGCCCgagaaCUggGGUCg -3' miRNA: 3'- -CGUCC-UUGUGCG---ACGGGa----GAaaCCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 55573 | 0.7 | 0.553096 |
Target: 5'- aGCAGGAACaucaccgccGCGCUGgCCacgcgauaccacgUCUcgcgcgccUUGGCCg -3' miRNA: 3'- -CGUCCUUG---------UGCGACgGG-------------AGA--------AACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 64444 | 0.7 | 0.554165 |
Target: 5'- uGCcGGG--GCGCUGCCCUUgg-GGCg -3' miRNA: 3'- -CGuCCUugUGCGACGGGAGaaaCCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 38583 | 0.7 | 0.575664 |
Target: 5'- uCAGGAugAUGCUGCCCgauccGGUg -3' miRNA: 3'- cGUCCUugUGCGACGGGagaaaCCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 50633 | 0.69 | 0.596258 |
Target: 5'- aGCGGuccaugcGAuUACGCcGCCCUCgg-GGCCa -3' miRNA: 3'- -CGUC-------CUuGUGCGaCGGGAGaaaCCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 20527 | 0.68 | 0.6519 |
Target: 5'- aGCAcGggUGCGCUGCCCgcagcgUGGUg -3' miRNA: 3'- -CGUcCuuGUGCGACGGGagaa--ACCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 42039 | 0.68 | 0.662797 |
Target: 5'- uCGGGucGACuGCGCUGUCCUCggggUGGgCg -3' miRNA: 3'- cGUCC--UUG-UGCGACGGGAGaa--ACCgG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 67441 | 0.68 | 0.673665 |
Target: 5'- cGCuuGAGCGCGUcggggaUGCCCUUUUcGGCg -3' miRNA: 3'- -CGucCUUGUGCG------ACGGGAGAAaCCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 28157 | 0.68 | 0.680168 |
Target: 5'- --cGGccucGACGCGCUGCCCUUgacguaguccggUGGCg -3' miRNA: 3'- cguCC----UUGUGCGACGGGAGaa----------ACCGg -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 26790 | 0.68 | 0.683413 |
Target: 5'- --cGGAACAccagcgcCGCcGCCCgg-UUGGCCg -3' miRNA: 3'- cguCCUUGU-------GCGaCGGGagaAACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 32528 | 0.68 | 0.684494 |
Target: 5'- gGCGGGcGGCAgGCuUGaCCUUgcugUUGGCCg -3' miRNA: 3'- -CGUCC-UUGUgCG-ACgGGAGa---AACCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 14865 | 0.68 | 0.695274 |
Target: 5'- cGCgAGGuuCGCGCUGCCCgagcc-GCCg -3' miRNA: 3'- -CG-UCCuuGUGCGACGGGagaaacCGG- -5' |
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23232 | 5' | -54.7 | NC_005259.1 | + | 44463 | 0.67 | 0.716643 |
Target: 5'- cCGGGGAUGcCGCcGCCCUggccgGGCCa -3' miRNA: 3'- cGUCCUUGU-GCGaCGGGAgaaa-CCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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