Results 1 - 20 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23235 | 3' | -62.7 | NC_005259.1 | + | 44688 | 0.66 | 0.42791 |
Target: 5'- gUGGC-CCGCauaUGGCCCgCGUCcCACcCg -3' miRNA: 3'- -ACCGuGGCGg--GCCGGG-GUAGaGUGcG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 1376 | 0.66 | 0.42791 |
Target: 5'- -cGCGCCGCCCgcgaGGCCCgCGcaugagccUCUCAUa- -3' miRNA: 3'- acCGUGGCGGG----CCGGG-GU--------AGAGUGcg -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 11444 | 0.66 | 0.42791 |
Target: 5'- gGGCcgcucgcuGCCGCCgGuGCCUauCGUCUCGCa- -3' miRNA: 3'- aCCG--------UGGCGGgC-CGGG--GUAGAGUGcg -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 4682 | 0.66 | 0.42791 |
Target: 5'- cUGGCucggGCCgacaGCUCGGCCCCGUggugcgaugaC-CGCGCc -3' miRNA: 3'- -ACCG----UGG----CGGGCCGGGGUA----------GaGUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 51217 | 0.66 | 0.42791 |
Target: 5'- cGGUagaucACCGCCggaucguaGGCCCgGUacgUCACGCc -3' miRNA: 3'- aCCG-----UGGCGGg-------CCGGGgUAg--AGUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 7429 | 0.66 | 0.419 |
Target: 5'- cGGCGgUGCCCGcGCCgCAUggCAgCGCc -3' miRNA: 3'- aCCGUgGCGGGC-CGGgGUAgaGU-GCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 27409 | 0.66 | 0.410205 |
Target: 5'- cGGCGgCaGCUCGGUgCCCGcgaggaauuggCUCACGCg -3' miRNA: 3'- aCCGUgG-CGGGCCG-GGGUa----------GAGUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 47765 | 0.66 | 0.410205 |
Target: 5'- cGGCugCGCgaUGGCCUUuuggaaaUCGCGCa -3' miRNA: 3'- aCCGugGCGg-GCCGGGGuag----AGUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 12321 | 0.66 | 0.401524 |
Target: 5'- gUGcGCACCGCC--GCCag--CUCACGCa -3' miRNA: 3'- -AC-CGUGGCGGgcCGGgguaGAGUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 3143 | 0.66 | 0.396373 |
Target: 5'- cGGCACCGCCCgugggacuacacGGUgaugCCCggcgaugucaaggucGUCggugCGCGCa -3' miRNA: 3'- aCCGUGGCGGG------------CCG----GGG---------------UAGa---GUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 53205 | 0.66 | 0.392962 |
Target: 5'- cGGCGaucagauucgcgUCGCCCGcGCCCUgcuugaggUUCGCGCc -3' miRNA: 3'- aCCGU------------GGCGGGC-CGGGGua------GAGUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 20490 | 0.66 | 0.392962 |
Target: 5'- cGaCACCGCCCGGCagCUCAUCg-GCGa -3' miRNA: 3'- aCcGUGGCGGGCCG--GGGUAGagUGCg -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 66258 | 0.66 | 0.384519 |
Target: 5'- -aGCGCgGCCCGcGCCgggCGgcggCUCACGCc -3' miRNA: 3'- acCGUGgCGGGC-CGGg--GUa---GAGUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 66745 | 0.66 | 0.376198 |
Target: 5'- aGGCugauGCUGCCCGGCCUgccaGUCgacCGCc -3' miRNA: 3'- aCCG----UGGCGGGCCGGGg---UAGaguGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 59905 | 0.66 | 0.376198 |
Target: 5'- uUGGUGCCGaCacaGuGCCCCGUCcauaccaagCACGCg -3' miRNA: 3'- -ACCGUGGCgGg--C-CGGGGUAGa--------GUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 4800 | 0.67 | 0.367998 |
Target: 5'- aGGUACUGCCCGaGCUgCCAgacgUUGCGCc -3' miRNA: 3'- aCCGUGGCGGGC-CGG-GGUag--AGUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 66184 | 0.67 | 0.367998 |
Target: 5'- cGGCACCGCugCCGGUgCaguacggGUCagGCGCg -3' miRNA: 3'- aCCGUGGCG--GGCCGgGg------UAGagUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 42640 | 0.67 | 0.359922 |
Target: 5'- aGGCACC--UCGGCCCCGaugugggCggcCACGCg -3' miRNA: 3'- aCCGUGGcgGGCCGGGGUa------Ga--GUGCG- -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 7279 | 0.67 | 0.351971 |
Target: 5'- cGGCuACCGCCCcGCCCCcaagCACu- -3' miRNA: 3'- aCCG-UGGCGGGcCGGGGuagaGUGcg -5' |
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23235 | 3' | -62.7 | NC_005259.1 | + | 9710 | 0.67 | 0.344145 |
Target: 5'- gGGCACCGuCCuCGGgCCgGUCgagCuCGCc -3' miRNA: 3'- aCCGUGGC-GG-GCCgGGgUAGa--GuGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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