Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23257 | 3' | -59.2 | NC_005259.1 | + | 1844 | 0.66 | 0.499595 |
Target: 5'- cGCUG-CCGCCGAGGCcgagaaAGCCgccgCcGCg- -3' miRNA: 3'- -UGACuGGUGGCUCCG------UCGGa---GuCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 6105 | 0.66 | 0.520126 |
Target: 5'- --aGAUCGCCGAGGCcgaGGCCgccGCUa -3' miRNA: 3'- ugaCUGGUGGCUCCG---UCGGaguCGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 8352 | 0.73 | 0.197622 |
Target: 5'- cGCUG-CUGCCGGuaucgcucggcGGCGGCCUCGGCa- -3' miRNA: 3'- -UGACuGGUGGCU-----------CCGUCGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 17254 | 0.66 | 0.489465 |
Target: 5'- cGCUGGCCACCucagacaAGGCAGUgCUCGaccGCUa -3' miRNA: 3'- -UGACUGGUGGc------UCCGUCG-GAGU---CGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 19379 | 0.66 | 0.519092 |
Target: 5'- cGCUGAUCACCGGccccaacGGCAGCgUCGa--- -3' miRNA: 3'- -UGACUGGUGGCU-------CCGUCGgAGUcgaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 20164 | 0.73 | 0.192491 |
Target: 5'- cCUGGCCACCGcgcccGCcGCCUCGGCUg -3' miRNA: 3'- uGACUGGUGGCuc---CGuCGGAGUCGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 21607 | 0.67 | 0.430875 |
Target: 5'- aGCUGAucuacaCCACCGAGGCuGUgUCGcGCg- -3' miRNA: 3'- -UGACU------GGUGGCUCCGuCGgAGU-CGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 23355 | 0.66 | 0.479432 |
Target: 5'- gGCUG-CCugCGAGGCcGCCgaGGCa- -3' miRNA: 3'- -UGACuGGugGCUCCGuCGGagUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 29390 | 0.67 | 0.430875 |
Target: 5'- cGCcGACCuacACCGAGG--GCCUCGGCg- -3' miRNA: 3'- -UGaCUGG---UGGCUCCguCGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 29768 | 0.67 | 0.430875 |
Target: 5'- gACUGAUCAgacccaCGAGGCAcccccgguGCCUCGGUa- -3' miRNA: 3'- -UGACUGGUg-----GCUCCGU--------CGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 30265 | 0.66 | 0.509817 |
Target: 5'- gACUGACaugCACCGGGccaGCAGCgUCgAGCUc -3' miRNA: 3'- -UGACUG---GUGGCUC---CGUCGgAG-UCGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 30684 | 0.76 | 0.121691 |
Target: 5'- aGCUGGCCACCuGAGGCAGCacgAGCg- -3' miRNA: 3'- -UGACUGGUGG-CUCCGUCGgagUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 31667 | 0.68 | 0.38182 |
Target: 5'- cUUGACCucgacgggguugcCCGAGGCAuCCUCGGCg- -3' miRNA: 3'- uGACUGGu------------GGCUCCGUcGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 33591 | 0.7 | 0.296919 |
Target: 5'- cGCgGGCCACCGAGGCgcGGUCg-AGCUg -3' miRNA: 3'- -UGaCUGGUGGCUCCG--UCGGagUCGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 34532 | 1.05 | 0.000759 |
Target: 5'- gACUGACCACCGAGGCAGCCUCAGCUUg -3' miRNA: 3'- -UGACUGGUGGCUCCGUCGGAGUCGAA- -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 35884 | 0.7 | 0.289712 |
Target: 5'- ---cGCCGCCGAGGCAGaCCaCGGCg- -3' miRNA: 3'- ugacUGGUGGCUCCGUC-GGaGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 36944 | 0.68 | 0.412274 |
Target: 5'- uGCUG-UCACCGGGGUGcccgcgagaccGCCUCGGCc- -3' miRNA: 3'- -UGACuGGUGGCUCCGU-----------CGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 40884 | 0.68 | 0.394177 |
Target: 5'- cACcGGCCACCGuGGCGGggUCGGCa- -3' miRNA: 3'- -UGaCUGGUGGCuCCGUCggAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 42539 | 0.68 | 0.412274 |
Target: 5'- -aUGGgCACCGAGGUGGCgggCAGCUc -3' miRNA: 3'- ugACUgGUGGCUCCGUCGga-GUCGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 45006 | 0.68 | 0.412274 |
Target: 5'- cGCcGACCGCCGGGGCcgcGCCgccCuGCUUc -3' miRNA: 3'- -UGaCUGGUGGCUCCGu--CGGa--GuCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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