Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23257 | 3' | -59.2 | NC_005259.1 | + | 51131 | 0.66 | 0.530516 |
Target: 5'- --aGAUCACCGucgGGGCGGCCaUgAGCg- -3' miRNA: 3'- ugaCUGGUGGC---UCCGUCGG-AgUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 40884 | 0.68 | 0.394177 |
Target: 5'- cACcGGCCACCGuGGCGGggUCGGCa- -3' miRNA: 3'- -UGaCUGGUGGCuCCGUCggAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 36944 | 0.68 | 0.412274 |
Target: 5'- uGCUG-UCACCGGGGUGcccgcgagaccGCCUCGGCc- -3' miRNA: 3'- -UGACuGGUGGCUCCGU-----------CGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 42539 | 0.68 | 0.412274 |
Target: 5'- -aUGGgCACCGAGGUGGCgggCAGCUc -3' miRNA: 3'- ugACUgGUGGCUCCGUCGga-GUCGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 45006 | 0.68 | 0.412274 |
Target: 5'- cGCcGACCGCCGGGGCcgcGCCgccCuGCUUc -3' miRNA: 3'- -UGaCUGGUGGCUCCGu--CGGa--GuCGAA- -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 45729 | 0.68 | 0.412274 |
Target: 5'- cCUGcgcACCACCGAGGCcgcgcaccGCCUCGGg-- -3' miRNA: 3'- uGAC---UGGUGGCUCCGu-------CGGAGUCgaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 60266 | 0.67 | 0.420583 |
Target: 5'- gGCUGGCCACCagcgcugccGGGCGGCCaucgucgUCGGUg- -3' miRNA: 3'- -UGACUGGUGGc--------UCCGUCGG-------AGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 48425 | 0.67 | 0.420583 |
Target: 5'- --cGACCGCCaccaccgaggcgcGAGGCAGCggCAGCg- -3' miRNA: 3'- ugaCUGGUGG-------------CUCCGUCGgaGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 29768 | 0.67 | 0.430875 |
Target: 5'- gACUGAUCAgacccaCGAGGCAcccccgguGCCUCGGUa- -3' miRNA: 3'- -UGACUGGUg-----GCUCCGU--------CGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 31667 | 0.68 | 0.38182 |
Target: 5'- cUUGACCucgacgggguugcCCGAGGCAuCCUCGGCg- -3' miRNA: 3'- uGACUGGu------------GGCUCCGUcGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 68623 | 0.68 | 0.368017 |
Target: 5'- gUUGGCCAgCCG-GGCAcCCUCGGCg- -3' miRNA: 3'- uGACUGGU-GGCuCCGUcGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 33591 | 0.7 | 0.296919 |
Target: 5'- cGCgGGCCACCGAGGCgcGGUCg-AGCUg -3' miRNA: 3'- -UGaCUGGUGGCUCCG--UCGGagUCGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 30684 | 0.76 | 0.121691 |
Target: 5'- aGCUGGCCACCuGAGGCAGCacgAGCg- -3' miRNA: 3'- -UGACUGGUGG-CUCCGUCGgagUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 49125 | 0.74 | 0.16811 |
Target: 5'- cGCUGAUCACCGGGGUcaagcuGGCCaagcagaUCGGCUa -3' miRNA: 3'- -UGACUGGUGGCUCCG------UCGG-------AGUCGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 20164 | 0.73 | 0.192491 |
Target: 5'- cCUGGCCACCGcgcccGCcGCCUCGGCUg -3' miRNA: 3'- uGACUGGUGGCuc---CGuCGGAGUCGAa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 8352 | 0.73 | 0.197622 |
Target: 5'- cGCUG-CUGCCGGuaucgcucggcGGCGGCCUCGGCa- -3' miRNA: 3'- -UGACuGGUGGCU-----------CCGUCGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 58781 | 0.72 | 0.202871 |
Target: 5'- cGCUGugCGCCggucGAGGCGgugauuGCCUCGGCg- -3' miRNA: 3'- -UGACugGUGG----CUCCGU------CGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 55033 | 0.71 | 0.249343 |
Target: 5'- cACgGGCUGCCGAGGUgcuuGCCUCGGUg- -3' miRNA: 3'- -UGaCUGGUGGCUCCGu---CGGAGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 35884 | 0.7 | 0.289712 |
Target: 5'- ---cGCCGCCGAGGCAGaCCaCGGCg- -3' miRNA: 3'- ugacUGGUGGCUCCGUC-GGaGUCGaa -5' |
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23257 | 3' | -59.2 | NC_005259.1 | + | 54693 | 0.7 | 0.289712 |
Target: 5'- --aGACCGugUCGAGGCggguGGCCUCAGCc- -3' miRNA: 3'- ugaCUGGU--GGCUCCG----UCGGAGUCGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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