Results 1 - 20 of 79 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23263 | 3' | -58.5 | NC_005259.1 | + | 32807 | 1.1 | 0.000698 |
Target: 5'- aGGUCGAUCCCGACGCACCGCCGUCGAu -3' miRNA: 3'- -CCAGCUAGGGCUGCGUGGCGGCAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 48390 | 0.78 | 0.135087 |
Target: 5'- uGUCGuugcCgCCGACGC-CCGCCGUCGAg -3' miRNA: 3'- cCAGCua--G-GGCUGCGuGGCGGCAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 22647 | 0.76 | 0.178244 |
Target: 5'- cGGUCGAcgccgcccaccacaUCgCCGACGCacugACCGCCGcCGAu -3' miRNA: 3'- -CCAGCU--------------AG-GGCUGCG----UGGCGGCaGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 58695 | 0.75 | 0.219759 |
Target: 5'- gGGUCGG-CUCGuCGcCGCCGCCGUCa- -3' miRNA: 3'- -CCAGCUaGGGCuGC-GUGGCGGCAGcu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 46868 | 0.74 | 0.236686 |
Target: 5'- cGGUCGAggCuuGAauCGCGCCGCCGcccgCGAu -3' miRNA: 3'- -CCAGCUa-GggCU--GCGUGGCGGCa---GCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 27512 | 0.74 | 0.236686 |
Target: 5'- aGGUCGA-CgUGucCGCACaCGCCGUCGAg -3' miRNA: 3'- -CCAGCUaGgGCu-GCGUG-GCGGCAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 42204 | 0.74 | 0.242567 |
Target: 5'- cGUCGcgauggaugCCCGcCGCGCCGCCGUCc- -3' miRNA: 3'- cCAGCua-------GGGCuGCGUGGCGGCAGcu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 66217 | 0.74 | 0.248569 |
Target: 5'- cGGUCGcg-CCGACGCACaCGCgGUCGu -3' miRNA: 3'- -CCAGCuagGGCUGCGUG-GCGgCAGCu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 30817 | 0.74 | 0.260941 |
Target: 5'- aGUCGGguagUCCGGCGCGCCGagGUCGGc -3' miRNA: 3'- cCAGCUa---GGGCUGCGUGGCggCAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 37722 | 0.74 | 0.267312 |
Target: 5'- ---aGA-CCCGAgGCACCGCCGcCGAc -3' miRNA: 3'- ccagCUaGGGCUgCGUGGCGGCaGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 45613 | 0.73 | 0.280432 |
Target: 5'- -cUCGGUCUCGAUGC-CCGCCGggcCGGg -3' miRNA: 3'- ccAGCUAGGGCUGCGuGGCGGCa--GCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 6253 | 0.73 | 0.285143 |
Target: 5'- gGGUCGAuaUCCCGcAC-CGCCGCCGcgccuaccucguggUCGAg -3' miRNA: 3'- -CCAGCU--AGGGC-UGcGUGGCGGC--------------AGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 17721 | 0.73 | 0.294058 |
Target: 5'- --cCGAUCaCCGACGCACCGUgGcugcUCGAc -3' miRNA: 3'- ccaGCUAG-GGCUGCGUGGCGgC----AGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 50680 | 0.73 | 0.294058 |
Target: 5'- cGUCGGUgCCGACgagGCGCUGCuCGUCGu -3' miRNA: 3'- cCAGCUAgGGCUG---CGUGGCG-GCAGCu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 18186 | 0.72 | 0.308195 |
Target: 5'- aGGUCGAcCCCGAcauCGCACCcaaGCagGUCGAc -3' miRNA: 3'- -CCAGCUaGGGCU---GCGUGG---CGg-CAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 26397 | 0.72 | 0.3221 |
Target: 5'- --gCGAUUCCGACGCcgggcgcggcgguGCCGCCGUaGAa -3' miRNA: 3'- ccaGCUAGGGCUGCG-------------UGGCGGCAgCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 18286 | 0.72 | 0.322845 |
Target: 5'- -cUCGAcgCCCGACGaGCCGCCGUUc- -3' miRNA: 3'- ccAGCUa-GGGCUGCgUGGCGGCAGcu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 41319 | 0.72 | 0.330363 |
Target: 5'- aGGUCGAcCUCGAaaaggGCGgUGCCGUCGGa -3' miRNA: 3'- -CCAGCUaGGGCUg----CGUgGCGGCAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 10736 | 0.71 | 0.353683 |
Target: 5'- cGGU-GAUCaCCG-CGCGCUGCCGggCGAu -3' miRNA: 3'- -CCAgCUAG-GGCuGCGUGGCGGCa-GCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 10543 | 0.71 | 0.353683 |
Target: 5'- gGGUCGAUCUucuggaugugcuCGAucaauCGCGCCGCC-UCGAc -3' miRNA: 3'- -CCAGCUAGG------------GCU-----GCGUGGCGGcAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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