Results 1 - 20 of 79 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23263 | 3' | -58.5 | NC_005259.1 | + | 425 | 0.66 | 0.638726 |
Target: 5'- cGUCGAggaUGACGCACCGaucuaCGUCa- -3' miRNA: 3'- cCAGCUaggGCUGCGUGGCg----GCAGcu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 5402 | 0.67 | 0.62834 |
Target: 5'- cGUCGucaucaCCCG-CGCugGCCGCCacGUCGAg -3' miRNA: 3'- cCAGCua----GGGCuGCG--UGGCGG--CAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 6253 | 0.73 | 0.285143 |
Target: 5'- gGGUCGAuaUCCCGcAC-CGCCGCCGcgccuaccucguggUCGAg -3' miRNA: 3'- -CCAGCU--AGGGC-UGcGUGGCGGC--------------AGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 6894 | 0.7 | 0.412477 |
Target: 5'- cGUCG--CCCGcaacgcACGCGCCGCCGccaUCGAg -3' miRNA: 3'- cCAGCuaGGGC------UGCGUGGCGGC---AGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 7308 | 0.67 | 0.607585 |
Target: 5'- -aUUGGgugCCCGAguuCACCGCCGUCGc -3' miRNA: 3'- ccAGCUa--GGGCUgc-GUGGCGGCAGCu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 9657 | 0.7 | 0.439457 |
Target: 5'- cGUCGGUaugCCCGuuguuccacAUGCGCUGCCgGUCGAg -3' miRNA: 3'- cCAGCUA---GGGC---------UGCGUGGCGG-CAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 9957 | 0.68 | 0.535913 |
Target: 5'- -aUCGAgccgaccgCCCGAC-CACCGCaccgGUCGAg -3' miRNA: 3'- ccAGCUa-------GGGCUGcGUGGCGg---CAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 10543 | 0.71 | 0.353683 |
Target: 5'- gGGUCGAUCUucuggaugugcuCGAucaauCGCGCCGCC-UCGAc -3' miRNA: 3'- -CCAGCUAGG------------GCU-----GCGUGGCGGcAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 10736 | 0.71 | 0.353683 |
Target: 5'- cGGU-GAUCaCCG-CGCGCUGCCGggCGAu -3' miRNA: 3'- -CCAgCUAG-GGCuGCGUGGCGGCa-GCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 12152 | 0.7 | 0.430352 |
Target: 5'- -cUUGAUCaccgCCGACgGCACCGCCcucgGUCGAa -3' miRNA: 3'- ccAGCUAG----GGCUG-CGUGGCGG----CAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 12199 | 0.68 | 0.566359 |
Target: 5'- uGGUCGuguagagaugCaCCGGCGCACCGCCc---- -3' miRNA: 3'- -CCAGCua--------G-GGCUGCGUGGCGGcagcu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 12316 | 0.7 | 0.448672 |
Target: 5'- uGUCGGUgcgcaCCGccgccagcucACGCACCGCCGccUCGAa -3' miRNA: 3'- cCAGCUAg----GGC----------UGCGUGGCGGC--AGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 16458 | 0.67 | 0.607585 |
Target: 5'- cGGcaUCGGUacCCCGcCGCAgaUCGCCGUCa- -3' miRNA: 3'- -CC--AGCUA--GGGCuGCGU--GGCGGCAGcu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 16688 | 0.66 | 0.669818 |
Target: 5'- cGG-CGAUCCUGG-GCACCGaggugaCCGcCGAg -3' miRNA: 3'- -CCaGCUAGGGCUgCGUGGC------GGCaGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 17359 | 0.69 | 0.457993 |
Target: 5'- aGGUCGGcggCUCGGCGCuggUCGUgGUCGAc -3' miRNA: 3'- -CCAGCUa--GGGCUGCGu--GGCGgCAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 17721 | 0.73 | 0.294058 |
Target: 5'- --cCGAUCaCCGACGCACCGUgGcugcUCGAc -3' miRNA: 3'- ccaGCUAG-GGCUGCGUGGCGgC----AGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 17885 | 0.68 | 0.525888 |
Target: 5'- cGGUUGGaCCCc-CGCACCGCgCGcCGAu -3' miRNA: 3'- -CCAGCUaGGGcuGCGUGGCG-GCaGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 18186 | 0.72 | 0.308195 |
Target: 5'- aGGUCGAcCCCGAcauCGCACCcaaGCagGUCGAc -3' miRNA: 3'- -CCAGCUaGGGCU---GCGUGG---CGg-CAGCU- -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 18286 | 0.72 | 0.322845 |
Target: 5'- -cUCGAcgCCCGACGaGCCGCCGUUc- -3' miRNA: 3'- ccAGCUa-GGGCUGCgUGGCGGCAGcu -5' |
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23263 | 3' | -58.5 | NC_005259.1 | + | 20087 | 0.71 | 0.369865 |
Target: 5'- cGUCGAaaccaccgaCCCGugGUgaGCCGCCGUCu- -3' miRNA: 3'- cCAGCUa--------GGGCugCG--UGGCGGCAGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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