Results 1 - 20 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23272 | 3' | -59.3 | NC_005259.1 | + | 7403 | 0.66 | 0.607825 |
Target: 5'- aCCCGCaACGGCauccucguCGccGUCGGCGGu -3' miRNA: 3'- cGGGCGaUGCCGgu------GCucUAGCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 27144 | 0.66 | 0.607825 |
Target: 5'- gGgCgGCUGCGGUguUGGGGccaacgaugccgUCGGCGAu -3' miRNA: 3'- -CgGgCGAUGCCGguGCUCU------------AGCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 43304 | 0.66 | 0.597393 |
Target: 5'- cGCCCGCcugaAUGGCUGCGuuGGccaccgccUUGGCGAu -3' miRNA: 3'- -CGGGCGa---UGCCGGUGCu-CU--------AGCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 32183 | 0.66 | 0.597393 |
Target: 5'- aGCUCGCUGCGcGCCuc--GGUCuGCGAc -3' miRNA: 3'- -CGGGCGAUGC-CGGugcuCUAGcCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 10322 | 0.66 | 0.586988 |
Target: 5'- cGUCaucggGCU-CGGCgACGGGcUCGGCGGc -3' miRNA: 3'- -CGGg----CGAuGCCGgUGCUCuAGCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 60157 | 0.66 | 0.586988 |
Target: 5'- -gCCGCcucaGCGGCCucACGGG--CGGCGAa -3' miRNA: 3'- cgGGCGa---UGCCGG--UGCUCuaGCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 29853 | 0.66 | 0.586988 |
Target: 5'- cGCUCGCUgucgggcaucgaACuGCCAccgcccgauguCGAGGUCGGCa- -3' miRNA: 3'- -CGGGCGA------------UGcCGGU-----------GCUCUAGCCGcu -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 31831 | 0.66 | 0.58076 |
Target: 5'- cGCCCGCgucgaucuucucgcGCGuGaCCGCGucAUUGGCGAu -3' miRNA: 3'- -CGGGCGa-------------UGC-C-GGUGCucUAGCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 41132 | 0.66 | 0.58076 |
Target: 5'- uGCCCGCgucGCGcaGCCucaggccccaugugcACGAGGUguacUGGCGAg -3' miRNA: 3'- -CGGGCGa--UGC--CGG---------------UGCUCUA----GCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 54132 | 0.66 | 0.576617 |
Target: 5'- cGCUCGUgaccagACGGUUuCGAGGccCGGCGAu -3' miRNA: 3'- -CGGGCGa-----UGCCGGuGCUCUa-GCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 5412 | 0.66 | 0.576617 |
Target: 5'- aCCCGCgcUGGCCGCcacgucGAGGUCuGCGc -3' miRNA: 3'- cGGGCGauGCCGGUG------CUCUAGcCGCu -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 4275 | 0.66 | 0.576617 |
Target: 5'- gGgCgGCUGCGcuGCCACGAGcacggacCGGCGGc -3' miRNA: 3'- -CgGgCGAUGC--CGGUGCUCua-----GCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 19955 | 0.66 | 0.576617 |
Target: 5'- cGUCCGCaACGGCgGC----UCGGCGGu -3' miRNA: 3'- -CGGGCGaUGCCGgUGcucuAGCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 50331 | 0.66 | 0.575582 |
Target: 5'- aGCUCGUcGCGGCCcuuguccucggucACGAuGUCGGUGu -3' miRNA: 3'- -CGGGCGaUGCCGG-------------UGCUcUAGCCGCu -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 960 | 0.66 | 0.566287 |
Target: 5'- aGCUCGCgcaacggcucaACGGCgACGAGAcacCGGCa- -3' miRNA: 3'- -CGGGCGa----------UGCCGgUGCUCUa--GCCGcu -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 58662 | 0.66 | 0.556006 |
Target: 5'- aGCCCGgUGCaccggGGUgcagcuuccauCGCGGGGUCGGCu- -3' miRNA: 3'- -CGGGCgAUG-----CCG-----------GUGCUCUAGCCGcu -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 56677 | 0.66 | 0.556006 |
Target: 5'- gGCUCGUugcgccacccaUACGGCUGCGAGGU-GGCc- -3' miRNA: 3'- -CGGGCG-----------AUGCCGGUGCUCUAgCCGcu -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 66879 | 0.67 | 0.545779 |
Target: 5'- cGCaCCGCgccACGGCUugauCGuGAaCGGCGGu -3' miRNA: 3'- -CG-GGCGa--UGCCGGu---GCuCUaGCCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 51136 | 0.67 | 0.539672 |
Target: 5'- -aCCGUcgggGCGGCCAUGAGcgCguucggguugaccguGGCGAg -3' miRNA: 3'- cgGGCGa---UGCCGGUGCUCuaG---------------CCGCU- -5' |
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23272 | 3' | -59.3 | NC_005259.1 | + | 24884 | 0.67 | 0.535614 |
Target: 5'- -aCCGCUAuCGaGUCAgcCGAGGaCGGCGAg -3' miRNA: 3'- cgGGCGAU-GC-CGGU--GCUCUaGCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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