Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23348 | 3' | -53.9 | NC_005259.1 | + | 8671 | 0.66 | 0.859022 |
Target: 5'- gCCAcGCGGGcaacGGGGCCGCCuCgAUGUc -3' miRNA: 3'- gGGU-UGUCCu---UCCUGGUGGuGgUACA- -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 48805 | 0.66 | 0.8507 |
Target: 5'- uCCCGcCgAGGAAGGuagaaaugACCACCAUCAc-- -3' miRNA: 3'- -GGGUuG-UCCUUCC--------UGGUGGUGGUaca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 39988 | 0.66 | 0.8507 |
Target: 5'- cCUCGACGGGcu-GAUCGCCGCCGa-- -3' miRNA: 3'- -GGGUUGUCCuucCUGGUGGUGGUaca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 30008 | 0.66 | 0.8507 |
Target: 5'- aCCaCGACaAGGu--GACCACCGCCggGg -3' miRNA: 3'- -GG-GUUG-UCCuucCUGGUGGUGGuaCa -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 23655 | 0.66 | 0.8507 |
Target: 5'- gCCGACGGcGAcggcuGGACCGCCgACCccGa -3' miRNA: 3'- gGGUUGUC-CUu----CCUGGUGG-UGGuaCa -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 47800 | 0.66 | 0.848159 |
Target: 5'- gUCCGACGGuGAGucgcgagaccucgcGGGCCACCGCUg--- -3' miRNA: 3'- -GGGUUGUC-CUU--------------CCUGGUGGUGGuaca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 27575 | 0.66 | 0.842152 |
Target: 5'- aCCGGCGGGAu--GCCguacuugucgcACCACCAUGc -3' miRNA: 3'- gGGUUGUCCUuccUGG-----------UGGUGGUACa -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 25021 | 0.66 | 0.824411 |
Target: 5'- aCCaCAAacacaaAGGA--GAUCACCACCAUGg -3' miRNA: 3'- -GG-GUUg-----UCCUucCUGGUGGUGGUACa -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 37280 | 0.66 | 0.824411 |
Target: 5'- gCCCGGCAGGAcuugcauggcGGugC-CCGCCggGUc -3' miRNA: 3'- -GGGUUGUCCUu---------CCugGuGGUGGuaCA- -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 14554 | 0.66 | 0.815237 |
Target: 5'- gUCCGGCAGGccgagcGGGGACCGgcgaaCACCAUc- -3' miRNA: 3'- -GGGUUGUCC------UUCCUGGUg----GUGGUAca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 7844 | 0.67 | 0.805873 |
Target: 5'- cCCCuuACGGGAcGGACCG-CGCCGa-- -3' miRNA: 3'- -GGGu-UGUCCUuCCUGGUgGUGGUaca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 27542 | 0.67 | 0.786617 |
Target: 5'- cCCCAcgGCGGGAuguuGcGGCCACCGauCCAUa- -3' miRNA: 3'- -GGGU--UGUCCUu---C-CUGGUGGU--GGUAca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 48099 | 0.67 | 0.780713 |
Target: 5'- gCCCGACGGGuccggucaggaaaccGAGcacaccgccucGACCACCGCCGc-- -3' miRNA: 3'- -GGGUUGUCC---------------UUC-----------CUGGUGGUGGUaca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 24109 | 0.67 | 0.776746 |
Target: 5'- aCCGAUGGGAccGGGcggggacuacACCGCCGCCGc-- -3' miRNA: 3'- gGGUUGUCCU--UCC----------UGGUGGUGGUaca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 26200 | 0.67 | 0.766728 |
Target: 5'- -gCGGCAGcauGG-CCGCCGCCGUGUu -3' miRNA: 3'- ggGUUGUCcuuCCuGGUGGUGGUACA- -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 1741 | 0.68 | 0.756572 |
Target: 5'- aCCAACGaGAgaaAGaGAgCACCGCCAUGg -3' miRNA: 3'- gGGUUGUcCU---UC-CUgGUGGUGGUACa -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 25058 | 0.68 | 0.756572 |
Target: 5'- gCCCGuuuuCAGGcAAGGguggcgcuGCCACCGCCGc-- -3' miRNA: 3'- -GGGUu---GUCC-UUCC--------UGGUGGUGGUaca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 60893 | 0.68 | 0.725399 |
Target: 5'- aCCGaauGCGGGcuuGGCCACCAUCGUGa -3' miRNA: 3'- gGGU---UGUCCuucCUGGUGGUGGUACa -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 4399 | 0.68 | 0.725399 |
Target: 5'- aCCGACAccgaacuacGAGGGCCGCCACCGc-- -3' miRNA: 3'- gGGUUGUcc-------UUCCUGGUGGUGGUaca -5' |
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23348 | 3' | -53.9 | NC_005259.1 | + | 355 | 0.68 | 0.713747 |
Target: 5'- aCCGaaaGCAGGAaacgaugAGcACCAUCACCAUGa -3' miRNA: 3'- gGGU---UGUCCU-------UCcUGGUGGUGGUACa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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