Results 1 - 20 of 75 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23376 | 5' | -60 | NC_005259.1 | + | 67207 | 0.86 | 0.024567 |
Target: 5'- uCGCCGCugucggcggggucgaUGCCGaCCACGGCGGUGUAGGg -3' miRNA: 3'- -GCGGCG---------------ACGGC-GGUGCUGUCGCAUCC- -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 19838 | 0.77 | 0.104714 |
Target: 5'- uCGCgGCUGCUGCCGCGcuCGGUGUGGc -3' miRNA: 3'- -GCGgCGACGGCGGUGCu-GUCGCAUCc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 49243 | 0.77 | 0.122902 |
Target: 5'- uCGaCCGCUGCCGUCACGAUuGCGUu-- -3' miRNA: 3'- -GC-GGCGACGGCGGUGCUGuCGCAucc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 35643 | 0.75 | 0.151721 |
Target: 5'- cCGCCGCUGCCGCUgGCGAgGGUGg--- -3' miRNA: 3'- -GCGGCGACGGCGG-UGCUgUCGCaucc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 26280 | 0.75 | 0.159831 |
Target: 5'- uCGCCGgUGCCGCCcuCGACGG-GUAcGGg -3' miRNA: 3'- -GCGGCgACGGCGGu-GCUGUCgCAU-CC- -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 38951 | 0.74 | 0.172728 |
Target: 5'- uGCCGCUGCCGCCgagagccugcACGAUGGCa---- -3' miRNA: 3'- gCGGCGACGGCGG----------UGCUGUCGcaucc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 37665 | 0.74 | 0.17723 |
Target: 5'- cCGCCGgUGCCGCCACGguuGGUGUAu- -3' miRNA: 3'- -GCGGCgACGGCGGUGCug-UCGCAUcc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 26947 | 0.73 | 0.204923 |
Target: 5'- aCGCCGCUGCuggaacgugcgcagCGCCGugccgaacuCGGCGGUGUAGa -3' miRNA: 3'- -GCGGCGACG--------------GCGGU---------GCUGUCGCAUCc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 38726 | 0.73 | 0.22823 |
Target: 5'- -aCCGCUGCCGCCGuacCGGCGGUGg--- -3' miRNA: 3'- gcGGCGACGGCGGU---GCUGUCGCaucc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 32549 | 0.72 | 0.239814 |
Target: 5'- uGCUGUUgGCCGCCcCGGCgAGCGcGGGg -3' miRNA: 3'- gCGGCGA-CGGCGGuGCUG-UCGCaUCC- -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 45345 | 0.72 | 0.245788 |
Target: 5'- gCGCCGCcuggGCCGCCGCG-UAGuCGacgGGGu -3' miRNA: 3'- -GCGGCGa---CGGCGGUGCuGUC-GCa--UCC- -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 28296 | 0.72 | 0.264453 |
Target: 5'- gGCCacGgUGCCGCCcuGCG-CAGCGUAGa -3' miRNA: 3'- gCGG--CgACGGCGG--UGCuGUCGCAUCc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 28221 | 0.71 | 0.270925 |
Target: 5'- gGUCG-UGCCGCCAcCGuCGGCGgcGGg -3' miRNA: 3'- gCGGCgACGGCGGU-GCuGUCGCauCC- -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 59299 | 0.71 | 0.277523 |
Target: 5'- cCuaCGCUGUCGUgGCGGCAGCGgcGa -3' miRNA: 3'- -GcgGCGACGGCGgUGCUGUCGCauCc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 27276 | 0.71 | 0.28425 |
Target: 5'- aCGCCGCcuugaGCCGCCGCuGCAcGCGccGGa -3' miRNA: 3'- -GCGGCGa----CGGCGGUGcUGU-CGCauCC- -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 18852 | 0.71 | 0.291105 |
Target: 5'- uCGCCGgUGCCGUgcaACGguuGCGcGCGUGGGa -3' miRNA: 3'- -GCGGCgACGGCGg--UGC---UGU-CGCAUCC- -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 8841 | 0.71 | 0.298088 |
Target: 5'- gCGCUGC-GCCGaCCACcACAGCGUGc- -3' miRNA: 3'- -GCGGCGaCGGC-GGUGcUGUCGCAUcc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 41706 | 0.7 | 0.312443 |
Target: 5'- gGCaUGCaGCCGCCGCauaccGGCGGCGgggAGGa -3' miRNA: 3'- gCG-GCGaCGGCGGUG-----CUGUCGCa--UCC- -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 45644 | 0.7 | 0.312443 |
Target: 5'- gCGCCGgUGCCGCCGUGaACAccgccguccGCGUAGu -3' miRNA: 3'- -GCGGCgACGGCGGUGC-UGU---------CGCAUCc -5' |
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23376 | 5' | -60 | NC_005259.1 | + | 60298 | 0.7 | 0.312443 |
Target: 5'- uCGUCGgUGCgGCCACGgACGGCGaaaccGGGc -3' miRNA: 3'- -GCGGCgACGgCGGUGC-UGUCGCa----UCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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