Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23391 | 3' | -50.7 | NC_005259.1 | + | 42531 | 0.79 | 0.328551 |
Target: 5'- cGGCCCCGAUGGGCAccGAGGuGGCGg -3' miRNA: 3'- aCUGGGGCUGCUCGUuuUUCC-UUGUg -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 32559 | 0.76 | 0.457126 |
Target: 5'- -cGCCCCGGCGAGCGc--GGGGAUAg -3' miRNA: 3'- acUGGGGCUGCUCGUuuuUCCUUGUg -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 64429 | 0.74 | 0.541585 |
Target: 5'- gGAUCgCCGACGAGCugcc-GGGGCGCu -3' miRNA: 3'- aCUGG-GGCUGCUCGuuuuuCCUUGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 23750 | 0.73 | 0.630606 |
Target: 5'- cGACCCCGACGccccGCAGAccGGcccCACa -3' miRNA: 3'- aCUGGGGCUGCu---CGUUUuuCCuu-GUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 48095 | 0.73 | 0.641843 |
Target: 5'- uUGAgCCCGACGGGUccggucagGAAAccGAGCACa -3' miRNA: 3'- -ACUgGGGCUGCUCG--------UUUUucCUUGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 43042 | 0.71 | 0.716339 |
Target: 5'- aGGCCgCCGAgcAGCGAAccgacggggucgguGAGGAACGCg -3' miRNA: 3'- aCUGG-GGCUgcUCGUUU--------------UUCCUUGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 37767 | 0.71 | 0.730453 |
Target: 5'- gUGGCCCCuGCGGGCAc----GAGCGCa -3' miRNA: 3'- -ACUGGGGcUGCUCGUuuuucCUUGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 10082 | 0.71 | 0.730453 |
Target: 5'- gGACgCCGACGAGUAGAccccGAGGc-CACc -3' miRNA: 3'- aCUGgGGCUGCUCGUUU----UUCCuuGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 41494 | 0.7 | 0.782889 |
Target: 5'- cGACCUCGGCGguggcgGGCAGAccguGGAucuGCACg -3' miRNA: 3'- aCUGGGGCUGC------UCGUUUuu--CCU---UGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 16381 | 0.7 | 0.796905 |
Target: 5'- -uGCgCCGACGAGCuccacaccauacGGGGGACGCa -3' miRNA: 3'- acUGgGGCUGCUCGuuu---------UUCCUUGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 13898 | 0.7 | 0.796905 |
Target: 5'- aGACCCCGACaaccaacaaccaCAGAAAGGAAC-Cg -3' miRNA: 3'- aCUGGGGCUGcuc---------GUUUUUCCUUGuG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 16238 | 0.7 | 0.799864 |
Target: 5'- cGcACCCCGGugcugcgcaccgacCGAGCAGAuauGGGAuCGCa -3' miRNA: 3'- aC-UGGGGCU--------------GCUCGUUUu--UCCUuGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 4121 | 0.69 | 0.828496 |
Target: 5'- cGGCCUCGACGuggccaagaccgucGGCGAGuucAAGGA-CGCg -3' miRNA: 3'- aCUGGGGCUGC--------------UCGUUU---UUCCUuGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 64902 | 0.69 | 0.831257 |
Target: 5'- gUGACacgagcgaCGGCGAGCGu-GAGGAugACg -3' miRNA: 3'- -ACUGgg------GCUGCUCGUuuUUCCUugUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 25598 | 0.69 | 0.840318 |
Target: 5'- --uCCUCGuuGCGGGCAGcGAGGAugACa -3' miRNA: 3'- acuGGGGC--UGCUCGUUuUUCCUugUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 66929 | 0.69 | 0.840318 |
Target: 5'- gGugCUCGAcCGGGguGAgcGGGACACc -3' miRNA: 3'- aCugGGGCU-GCUCguUUuuCCUUGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 65689 | 0.69 | 0.857747 |
Target: 5'- cGG-CCCGACGAGCGGGccgaguaggacGAGGu-CACg -3' miRNA: 3'- aCUgGGGCUGCUCGUUU-----------UUCCuuGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 29406 | 0.68 | 0.866096 |
Target: 5'- gGGCCUCGGCGAGCucgu-GcGcGCACa -3' miRNA: 3'- aCUGGGGCUGCUCGuuuuuC-CuUGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 5641 | 0.68 | 0.874192 |
Target: 5'- cGugCCCGGCGAGgAc--GGcGGGCGCu -3' miRNA: 3'- aCugGGGCUGCUCgUuuuUC-CUUGUG- -5' |
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23391 | 3' | -50.7 | NC_005259.1 | + | 24417 | 0.68 | 0.874192 |
Target: 5'- aUGACCUCGGCGAGgAAGcgacAGcGGGCAa -3' miRNA: 3'- -ACUGGGGCUGCUCgUUUu---UC-CUUGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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