Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23391 | 3' | -50.7 | NC_005259.1 | + | 10082 | 0.71 | 0.730453 |
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Target: 5'- gGACgCCGACGAGUAGAccccGAGGc-CACc -3' miRNA: 3'- aCUGgGGCUGCUCGUUU----UUCCuuGUG- -5' |
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| 23391 | 3' | -50.7 | NC_005259.1 | + | 37767 | 0.71 | 0.730453 |
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Target: 5'- gUGGCCCCuGCGGGCAc----GAGCGCa -3' miRNA: 3'- -ACUGGGGcUGCUCGUuuuucCUUGUG- -5' |
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| 23391 | 3' | -50.7 | NC_005259.1 | + | 43042 | 0.71 | 0.716339 |
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Target: 5'- aGGCCgCCGAgcAGCGAAccgacggggucgguGAGGAACGCg -3' miRNA: 3'- aCUGG-GGCUgcUCGUUU--------------UUCCUUGUG- -5' |
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| 23391 | 3' | -50.7 | NC_005259.1 | + | 48095 | 0.73 | 0.641843 |
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Target: 5'- uUGAgCCCGACGGGUccggucagGAAAccGAGCACa -3' miRNA: 3'- -ACUgGGGCUGCUCG--------UUUUucCUUGUG- -5' |
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| 23391 | 3' | -50.7 | NC_005259.1 | + | 23750 | 0.73 | 0.630606 |
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Target: 5'- cGACCCCGACGccccGCAGAccGGcccCACa -3' miRNA: 3'- aCUGGGGCUGCu---CGUUUuuCCuu-GUG- -5' |
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| 23391 | 3' | -50.7 | NC_005259.1 | + | 64429 | 0.74 | 0.541585 |
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Target: 5'- gGAUCgCCGACGAGCugcc-GGGGCGCu -3' miRNA: 3'- aCUGG-GGCUGCUCGuuuuuCCUUGUG- -5' |
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| 23391 | 3' | -50.7 | NC_005259.1 | + | 32559 | 0.76 | 0.457126 |
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Target: 5'- -cGCCCCGGCGAGCGc--GGGGAUAg -3' miRNA: 3'- acUGGGGCUGCUCGUuuuUCCUUGUg -5' |
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| 23391 | 3' | -50.7 | NC_005259.1 | + | 42531 | 0.79 | 0.328551 |
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Target: 5'- cGGCCCCGAUGGGCAccGAGGuGGCGg -3' miRNA: 3'- aCUGGGGCUGCUCGUuuUUCC-UUGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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