Results 1 - 20 of 52 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23423 | 3' | -60.7 | NC_005259.1 | + | 58564 | 0.66 | 0.545188 |
Target: 5'- cGgGCGGuUGGGCcgg-AUCGCGgcGCGGCu -3' miRNA: 3'- -CgCGCC-GCCCGuaggUAGCGC--UGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 14567 | 0.66 | 0.545188 |
Target: 5'- aGCGgGGaCcGGCGaacaCCAUCGCGcUGGCc -3' miRNA: 3'- -CGCgCC-GcCCGUa---GGUAGCGCuGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 49591 | 0.66 | 0.53915 |
Target: 5'- gGCGgGGUcagcaccucgaccucGGGCAgcucaUCAUCGUcaucGGCGGCa -3' miRNA: 3'- -CGCgCCG---------------CCCGUa----GGUAGCG----CUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 33586 | 0.66 | 0.535137 |
Target: 5'- cGCGUcGCGGGCcaCCGagGCG-CGGUc -3' miRNA: 3'- -CGCGcCGCCCGuaGGUagCGCuGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 40120 | 0.66 | 0.535137 |
Target: 5'- uGCGCucguggaauuGGCGGGCGagcucgCCGUCGguUGuCGGUg -3' miRNA: 3'- -CGCG----------CCGCCCGUa-----GGUAGC--GCuGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 50658 | 0.66 | 0.525151 |
Target: 5'- uCGgGGCcauaGGGCA-CCAgCGCGuCGGUg -3' miRNA: 3'- cGCgCCG----CCCGUaGGUaGCGCuGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 944 | 0.66 | 0.515237 |
Target: 5'- -aGCGGaaaaguCGGGCAgCUcgCGCaACGGCu -3' miRNA: 3'- cgCGCC------GCCCGUaGGuaGCGcUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 66124 | 0.66 | 0.515237 |
Target: 5'- cGCGCGuCGaGCAggCCAUCa-GACGGCg -3' miRNA: 3'- -CGCGCcGCcCGUa-GGUAGcgCUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 48448 | 0.66 | 0.51425 |
Target: 5'- gGCaGCGGCaGcGCGUUCcaccgcgcgccggGUCGCGuCGGCc -3' miRNA: 3'- -CG-CGCCGcC-CGUAGG-------------UAGCGCuGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 29129 | 0.66 | 0.505399 |
Target: 5'- aGCGCGGC-GGUAUUCGccUCGCucaccgcgcuCGGCu -3' miRNA: 3'- -CGCGCCGcCCGUAGGU--AGCGcu--------GCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 44908 | 0.66 | 0.505399 |
Target: 5'- cGCGCuGUGGGCcgCCGcauaCGCGcugaucggguccAUGGCa -3' miRNA: 3'- -CGCGcCGCCCGuaGGUa---GCGC------------UGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 29964 | 0.66 | 0.505399 |
Target: 5'- gGUGgGGCGGGCGgugaCCGUccaacggacccCGC-ACGGUg -3' miRNA: 3'- -CGCgCCGCCCGUa---GGUA-----------GCGcUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 18780 | 0.66 | 0.505399 |
Target: 5'- uCGCGGUGGGCAa-----GCGGUGGCa -3' miRNA: 3'- cGCGCCGCCCGUagguagCGCUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 17538 | 0.66 | 0.505399 |
Target: 5'- aGCGaCGGCGGGCAUuucuacuucaCCGUgCcCGACGa- -3' miRNA: 3'- -CGC-GCCGCCCGUA----------GGUA-GcGCUGCcg -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 15588 | 0.66 | 0.495643 |
Target: 5'- aGCGCGaCGGGCcgCaugaGCuACGGCa -3' miRNA: 3'- -CGCGCcGCCCGuaGguagCGcUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 50245 | 0.66 | 0.495643 |
Target: 5'- gGCGCGGaucguggccaaCGGGCuacucgCUGUCGuCGGUGGCg -3' miRNA: 3'- -CGCGCC-----------GCCCGua----GGUAGC-GCUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 32878 | 0.66 | 0.493702 |
Target: 5'- cGCGCGGUgaucgauucaccggGGGCGagggugagCCAcccggcccgcucgggCGCGGCGGUa -3' miRNA: 3'- -CGCGCCG--------------CCCGUa-------GGUa--------------GCGCUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 56027 | 0.67 | 0.476395 |
Target: 5'- uCGCcgGGCGGGacuUCC-UCG-GGCGGCu -3' miRNA: 3'- cGCG--CCGCCCgu-AGGuAGCgCUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 4450 | 0.67 | 0.467854 |
Target: 5'- cGUGCGaGUGGGCAgcguaugggccaaggCCAUCG--GCGGUg -3' miRNA: 3'- -CGCGC-CGCCCGUa--------------GGUAGCgcUGCCG- -5' |
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23423 | 3' | -60.7 | NC_005259.1 | + | 63552 | 0.67 | 0.439062 |
Target: 5'- uGCuCGGCGGcCA-CCGaCGCGACGaGCg -3' miRNA: 3'- -CGcGCCGCCcGUaGGUaGCGCUGC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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