Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23447 | 3' | -51.2 | NC_005259.1 | + | 28931 | 0.66 | 0.951925 |
Target: 5'- aGCCAGaagcaGGCgGCACGAuccugcgccgcaUCGCCGAgguCGGu -3' miRNA: 3'- -CGGUUaa---CCG-CGUGCU------------AGUGGUU---GCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 16509 | 0.66 | 0.937555 |
Target: 5'- gGCCGAgagcgaGGCGCuGCGAgcgUCGCuCAAgGGu -3' miRNA: 3'- -CGGUUaa----CCGCG-UGCU---AGUG-GUUgCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 6355 | 0.66 | 0.937555 |
Target: 5'- uGCCGuacGuGCGCACGuuGUCGCCGggcGCGa -3' miRNA: 3'- -CGGUuaaC-CGCGUGC--UAGUGGU---UGCc -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 47943 | 0.66 | 0.956174 |
Target: 5'- aGCCGAUgacggcGGUGC-CGAUgGCCGccGCGc -3' miRNA: 3'- -CGGUUAa-----CCGCGuGCUAgUGGU--UGCc -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 20676 | 0.66 | 0.956174 |
Target: 5'- cGCCAugaugUGGuCGCACGGcacgCGCuCggUGGu -3' miRNA: 3'- -CGGUua---ACC-GCGUGCUa---GUG-GuuGCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 43784 | 0.66 | 0.956174 |
Target: 5'- cGCCGGgcaUGaGCaGCGCGggCACCGAguccuugcCGGg -3' miRNA: 3'- -CGGUUa--AC-CG-CGUGCuaGUGGUU--------GCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 6433 | 0.66 | 0.951925 |
Target: 5'- cGCCGAUgucGGCaccgucGCGCuGAUCGgCAGCGu -3' miRNA: 3'- -CGGUUAa--CCG------CGUG-CUAGUgGUUGCc -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 13790 | 0.66 | 0.947407 |
Target: 5'- -aCGAgcagUGGUGCGacAUCACCGGCGa -3' miRNA: 3'- cgGUUa---ACCGCGUgcUAGUGGUUGCc -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 51662 | 0.66 | 0.947407 |
Target: 5'- uGCCGcgcgGGCGCGuCGggCACgGGCGc -3' miRNA: 3'- -CGGUuaa-CCGCGU-GCuaGUGgUUGCc -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 48713 | 0.66 | 0.951925 |
Target: 5'- gGCUAucgcacgGGCGCGCGGaaacucguUUACgCAAUGGg -3' miRNA: 3'- -CGGUuaa----CCGCGUGCU--------AGUG-GUUGCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 55052 | 0.66 | 0.942618 |
Target: 5'- uGCCucggUGGCagucuGCGCGAg-AUCAGCGGc -3' miRNA: 3'- -CGGuua-ACCG-----CGUGCUagUGGUUGCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 778 | 0.66 | 0.942618 |
Target: 5'- cGCCGAggcGGCugcccucgGUugGGUgGCCAAgGGg -3' miRNA: 3'- -CGGUUaa-CCG--------CGugCUAgUGGUUgCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 50094 | 0.66 | 0.942618 |
Target: 5'- cGCCGA-UGGUGCGCccgaGGUUGCCcguacgcACGGg -3' miRNA: 3'- -CGGUUaACCGCGUG----CUAGUGGu------UGCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 8284 | 0.67 | 0.908068 |
Target: 5'- aCCg---GGUGCGCGggCACCcgcACGGa -3' miRNA: 3'- cGGuuaaCCGCGUGCuaGUGGu--UGCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 63388 | 0.67 | 0.914521 |
Target: 5'- cGCCGA-UGGCcCACGccgACCAAUGGu -3' miRNA: 3'- -CGGUUaACCGcGUGCuagUGGUUGCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 13411 | 0.67 | 0.908068 |
Target: 5'- gGCCuuccUGGC-CGgGGUCGCCGcCGGg -3' miRNA: 3'- -CGGuua-ACCGcGUgCUAGUGGUuGCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 38760 | 0.67 | 0.914521 |
Target: 5'- gGCCGGUgaGCuGCGCGAUCAugUCGGCGa -3' miRNA: 3'- -CGGUUAacCG-CGUGCUAGU--GGUUGCc -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 58388 | 0.67 | 0.914521 |
Target: 5'- uCCGAcgGuGCGCACcagcCACCGGCGGc -3' miRNA: 3'- cGGUUaaC-CGCGUGcua-GUGGUUGCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 28019 | 0.67 | 0.914521 |
Target: 5'- cGCCGGgguuGCGCGCGuUgGCCAgccACGGc -3' miRNA: 3'- -CGGUUaac-CGCGUGCuAgUGGU---UGCC- -5' |
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23447 | 3' | -51.2 | NC_005259.1 | + | 47081 | 0.67 | 0.926595 |
Target: 5'- cGCCGAgacgaugGGCaGCACGAucUCGCCccACGu -3' miRNA: 3'- -CGGUUaa-----CCG-CGUGCU--AGUGGu-UGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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