Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23454 | 5' | -56.8 | NC_005259.1 | + | 26020 | 0.66 | 0.621956 |
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Target: 5'- -gGAUGGCCGCaaGCGgGUUGGCcagcagcgccgccGGGUu -3' miRNA: 3'- aaCUACCGGCGcgUGU-UAACCG-------------CCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 25747 | 0.68 | 0.548478 |
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Target: 5'- -gGAccGCCGCGCACAcuauaccgcuauGCGGGCa -3' miRNA: 3'- aaCUacCGGCGCGUGUuaac--------CGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 18763 | 0.66 | 0.623046 |
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Target: 5'- -cGAcaUGGCCuGC-CACAucgcGGUGGGCa -3' miRNA: 3'- aaCU--ACCGG-CGcGUGUuaa-CCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 15394 | 0.69 | 0.464938 |
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Target: 5'- --cGUGGCCGCGCACucccaagGGUGcGCu -3' miRNA: 3'- aacUACCGGCGCGUGuuaa---CCGCcCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 12016 | 0.72 | 0.314619 |
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Target: 5'- -cGGUGGCCuuGUACGGU--GCGGGCg -3' miRNA: 3'- aaCUACCGGcgCGUGUUAacCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 8948 | 0.71 | 0.346458 |
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Target: 5'- -cGGUGGgCGCGC-CG--UGGaCGGGCa -3' miRNA: 3'- aaCUACCgGCGCGuGUuaACC-GCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 8640 | 0.67 | 0.601266 |
|
Target: 5'- -aGaAUGGCgG-GCACGAggcugUGGuCGGGCu -3' miRNA: 3'- aaC-UACCGgCgCGUGUUa----ACC-GCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 4991 | 0.68 | 0.526277 |
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Target: 5'- -cGAUGGCCGgGCACuac-GGCaaGGUg -3' miRNA: 3'- aaCUACCGGCgCGUGuuaaCCGc-CCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 3088 | 0.71 | 0.38054 |
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Target: 5'- -aGAgaGCCGCGCAgCGAUgaGCGGGCg -3' miRNA: 3'- aaCUacCGGCGCGU-GUUAacCGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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