Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23454 | 5' | -56.8 | NC_005259.1 | + | 32359 | 0.7 | 0.407531 |
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Target: 5'- gUUGAUGaccGCCGUGUAg----GGCGGGCa -3' miRNA: 3'- -AACUAC---CGGCGCGUguuaaCCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 29953 | 0.7 | 0.407531 |
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Target: 5'- ----cGGUCGCgGCugGuggGGCGGGCg -3' miRNA: 3'- aacuaCCGGCG-CGugUuaaCCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 35662 | 0.7 | 0.407531 |
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Target: 5'- -gGGUGGCCaGCGCGg---UGGUGGuGCa -3' miRNA: 3'- aaCUACCGG-CGCGUguuaACCGCC-CG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 55709 | 0.7 | 0.435689 |
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Target: 5'- gUUGAUGGCCGCGaGCAccucgcccuuGUUGGCaaGCu -3' miRNA: 3'- -AACUACCGGCGCgUGU----------UAACCGccCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 63865 | 0.69 | 0.445322 |
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Target: 5'- -aGGUGGCCG-GCugGccUUGcGUGGGCg -3' miRNA: 3'- aaCUACCGGCgCGugUu-AAC-CGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 15394 | 0.69 | 0.464938 |
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Target: 5'- --cGUGGCCGCGCACucccaagGGUGcGCu -3' miRNA: 3'- aacUACCGGCGCGUGuuaa---CCGCcCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 47961 | 0.69 | 0.474914 |
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Target: 5'- -cGAUGGCCGcCGCGCcgcccgcgAAUaUGcCGGGCa -3' miRNA: 3'- aaCUACCGGC-GCGUG--------UUA-ACcGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 40108 | 0.69 | 0.495177 |
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Target: 5'- -cGAgucGGCgaaUGCGCucguGgAAUUGGCGGGCg -3' miRNA: 3'- aaCUa--CCG---GCGCG----UgUUAACCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 41869 | 0.66 | 0.666623 |
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Target: 5'- -cGAcgGGCa--GCACcg-UGGCGGGCc -3' miRNA: 3'- aaCUa-CCGgcgCGUGuuaACCGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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