Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23454 | 5' | -56.8 | NC_005259.1 | + | 43918 | 0.66 | 0.641587 |
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Target: 5'- -cGAUGGgcagcgacgacaCCGCGCuacugacagcgucgGCGAUgGGCGGGg -3' miRNA: 3'- aaCUACC------------GGCGCG--------------UGUUAaCCGCCCg -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 44480 | 0.66 | 0.633953 |
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Target: 5'- ---cUGGCCGgGC-CAGUUGGUgacguagaccgGGGUg -3' miRNA: 3'- aacuACCGGCgCGuGUUAACCG-----------CCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 47961 | 0.69 | 0.474914 |
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Target: 5'- -cGAUGGCCGcCGCGCcgcccgcgAAUaUGcCGGGCa -3' miRNA: 3'- aaCUACCGGC-GCGUG--------UUA-ACcGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 51015 | 0.72 | 0.322367 |
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Target: 5'- -gGAcguUGGUCGUGCuaccgACGAgcGGCGGGCa -3' miRNA: 3'- aaCU---ACCGGCGCG-----UGUUaaCCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 51416 | 0.67 | 0.558083 |
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Target: 5'- -cGAggGGCCG-GUugAGgucuugGGCGGGCc -3' miRNA: 3'- aaCUa-CCGGCgCGugUUaa----CCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 55293 | 0.66 | 0.63068 |
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Target: 5'- aUUGAgggacguuUGGCCGCGUgcgACGAccacagggguggucUUGGCGGcaGCg -3' miRNA: 3'- -AACU--------ACCGGCGCG---UGUU--------------AACCGCC--CG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 55709 | 0.7 | 0.435689 |
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Target: 5'- gUUGAUGGCCGCGaGCAccucgcccuuGUUGGCaaGCu -3' miRNA: 3'- -AACUACCGGCGCgUGU----------UAACCGccCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 63865 | 0.69 | 0.445322 |
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Target: 5'- -aGGUGGCCG-GCugGccUUGcGUGGGCg -3' miRNA: 3'- aaCUACCGGCgCGugUu-AAC-CGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 64889 | 0.67 | 0.568811 |
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Target: 5'- gUUGAUGaGCCGCGUgacACGAgcgacGGCGaGCg -3' miRNA: 3'- -AACUAC-CGGCGCG---UGUUaa---CCGCcCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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