Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23454 | 5' | -56.8 | NC_005259.1 | + | 55709 | 0.7 | 0.435689 |
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Target: 5'- gUUGAUGGCCGCGaGCAccucgcccuuGUUGGCaaGCu -3' miRNA: 3'- -AACUACCGGCGCgUGU----------UAACCGccCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 35662 | 0.7 | 0.407531 |
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Target: 5'- -gGGUGGCCaGCGCGg---UGGUGGuGCa -3' miRNA: 3'- aaCUACCGG-CGCGUguuaACCGCC-CG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 29953 | 0.7 | 0.407531 |
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Target: 5'- ----cGGUCGCgGCugGuggGGCGGGCg -3' miRNA: 3'- aacuaCCGGCG-CGugUuaaCCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 32359 | 0.7 | 0.407531 |
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Target: 5'- gUUGAUGaccGCCGUGUAg----GGCGGGCa -3' miRNA: 3'- -AACUAC---CGGCGCGUguuaaCCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 3088 | 0.71 | 0.38054 |
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Target: 5'- -aGAgaGCCGCGCAgCGAUgaGCGGGCg -3' miRNA: 3'- aaCUacCGGCGCGU-GUUAacCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 8948 | 0.71 | 0.346458 |
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Target: 5'- -cGGUGGgCGCGC-CG--UGGaCGGGCa -3' miRNA: 3'- aaCUACCgGCGCGuGUuaACC-GCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 51015 | 0.72 | 0.322367 |
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Target: 5'- -gGAcguUGGUCGUGCuaccgACGAgcGGCGGGCa -3' miRNA: 3'- aaCU---ACCGGCGCG-----UGUUaaCCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 12016 | 0.72 | 0.314619 |
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Target: 5'- -cGGUGGCCuuGUACGGU--GCGGGCg -3' miRNA: 3'- aaCUACCGGcgCGUGUUAacCGCCCG- -5' |
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| 23454 | 5' | -56.8 | NC_005259.1 | + | 41634 | 0.74 | 0.244858 |
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Target: 5'- aUGGUGGCgGUGC-CGGUcuGCGGGCg -3' miRNA: 3'- aACUACCGgCGCGuGUUAacCGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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