miRNA display CGI


Results 21 - 26 of 26 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23460 3' -60.3 NC_005259.1 + 15793 0.67 0.479298
Target:  5'- uGGC-CGCCGGGaucgaccGCCCGACaUCcaAGGUg -3'
miRNA:   3'- -CCGcGCGGCCUga-----UGGGCUG-AG--UCCA- -5'
23460 3' -60.3 NC_005259.1 + 68873 0.66 0.498722
Target:  5'- -cCGCGCCGGGC-GCgCCGAUg-AGGUa -3'
miRNA:   3'- ccGCGCGGCCUGaUG-GGCUGagUCCA- -5'
23460 3' -60.3 NC_005259.1 + 38027 0.66 0.508565
Target:  5'- aGGCccGCGuuGGugUACCCGGC-CAc-- -3'
miRNA:   3'- -CCG--CGCggCCugAUGGGCUGaGUcca -5'
23460 3' -60.3 NC_005259.1 + 67425 0.66 0.508565
Target:  5'- cGGgGUGCCGGGCUcacGCuuGAgcgcgUCGGGg -3'
miRNA:   3'- -CCgCGCGGCCUGA---UGggCUg----AGUCCa -5'
23460 3' -60.3 NC_005259.1 + 61506 0.66 0.548693
Target:  5'- aGGCGC-UCGGugUGCCgguCGACcucgUCGGGa -3'
miRNA:   3'- -CCGCGcGGCCugAUGG---GCUG----AGUCCa -5'
23460 3' -60.3 NC_005259.1 + 7448 0.66 0.558887
Target:  5'- uGGCaGCGCCGaggauggcuuGACUugacaauCCCGACauaggCGGGUa -3'
miRNA:   3'- -CCG-CGCGGC----------CUGAu------GGGCUGa----GUCCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.