Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23464 | 3' | -57.4 | NC_005259.1 | + | 19172 | 0.66 | 0.730011 |
Target: 5'- gUUCGUCGGCGCucaaCGGUGcccucgacaGCGAgCUCGu -3' miRNA: 3'- gGAGCAGCCGUG----GCCGC---------UGCUaGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 13455 | 0.66 | 0.719863 |
Target: 5'- --cCGUCGGCG-CGGCGcUGGUgCUCAc -3' miRNA: 3'- ggaGCAGCCGUgGCCGCuGCUA-GAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 63933 | 0.66 | 0.709635 |
Target: 5'- aCUCGUgGGUcuuaccguCCGGCaGCGAgaUCUCGg -3' miRNA: 3'- gGAGCAgCCGu-------GGCCGcUGCU--AGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 17524 | 0.66 | 0.709635 |
Target: 5'- gCUCGUgGGCACaCaGCGACGG-CgggCAu -3' miRNA: 3'- gGAGCAgCCGUG-GcCGCUGCUaGa--GU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 31887 | 0.66 | 0.678576 |
Target: 5'- aCCUCGcCGGUcucaaggcCCGGCG-CGAgcgcCUCAa -3' miRNA: 3'- -GGAGCaGCCGu-------GGCCGCuGCUa---GAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 61431 | 0.67 | 0.647153 |
Target: 5'- cCCUUG-CGggcGCGCCGGU--CGAUCUCAu -3' miRNA: 3'- -GGAGCaGC---CGUGGCCGcuGCUAGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 58913 | 0.67 | 0.668129 |
Target: 5'- gCUCGUCGGCGgucuugucgUCGGCGggcGCGGUggCAg -3' miRNA: 3'- gGAGCAGCCGU---------GGCCGC---UGCUAgaGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 58386 | 0.67 | 0.657652 |
Target: 5'- gCUcCGaCGGUgcgcaccagccACCGGCGGCGAUCa-- -3' miRNA: 3'- gGA-GCaGCCG-----------UGGCCGCUGCUAGagu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 64146 | 0.67 | 0.651354 |
Target: 5'- gCC-CGUCGGCGaugcccuugacguagUCGGCGACuGUCUg- -3' miRNA: 3'- -GGaGCAGCCGU---------------GGCCGCUGcUAGAgu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 51781 | 0.67 | 0.636642 |
Target: 5'- gCCUUGagguauUCGGCGCuCGGCGGguCGGUCagcUCAg -3' miRNA: 3'- -GGAGC------AGCCGUG-GCCGCU--GCUAG---AGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 20059 | 0.67 | 0.636642 |
Target: 5'- --aUGUCcGCugCGGUGGCGGUCgUCAc -3' miRNA: 3'- ggaGCAGcCGugGCCGCUGCUAG-AGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 62689 | 0.67 | 0.626126 |
Target: 5'- uCCUCGagugCGGUgucccauCCGGCGAUcaggcggggGAUCUCGc -3' miRNA: 3'- -GGAGCa---GCCGu------GGCCGCUG---------CUAGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 55333 | 0.67 | 0.626126 |
Target: 5'- gUCUUGgCGGCAgCGGUGGCGAUg--- -3' miRNA: 3'- -GGAGCaGCCGUgGCCGCUGCUAgagu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 8368 | 0.67 | 0.615616 |
Target: 5'- gCUCGgcggcggccUCGGCAUCGGCGA-GAgcCUCGg -3' miRNA: 3'- gGAGC---------AGCCGUGGCCGCUgCUa-GAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 12021 | 0.68 | 0.584195 |
Target: 5'- gCCUUGUaCGGUGCgGGCGGCaaccGGUaCUCAc -3' miRNA: 3'- -GGAGCA-GCCGUGgCCGCUG----CUA-GAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 7804 | 0.68 | 0.563418 |
Target: 5'- uUCUCGUuuguaaugacCGGUAaugacCCGGCGGCGGUCa-- -3' miRNA: 3'- -GGAGCA----------GCCGU-----GGCCGCUGCUAGagu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 56655 | 0.68 | 0.605119 |
Target: 5'- -aUCGagGGCACCGcGCGucACGggCUCGu -3' miRNA: 3'- ggAGCagCCGUGGC-CGC--UGCuaGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 15645 | 0.68 | 0.553102 |
Target: 5'- gCC-CGUCcgauccuguGCGacgaCGGCGACGGUCUCAc -3' miRNA: 3'- -GGaGCAGc--------CGUg---GCCGCUGCUAGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 42644 | 0.68 | 0.547965 |
Target: 5'- aCCUCGgccccgaugugggCGGcCACgCGGCugcuguuGGCGAUCUCGa -3' miRNA: 3'- -GGAGCa------------GCC-GUG-GCCG-------CUGCUAGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 22387 | 0.68 | 0.605119 |
Target: 5'- cCCg-GUCGGCcUCGGCGACGugaUCGa -3' miRNA: 3'- -GGagCAGCCGuGGCCGCUGCuagAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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