Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23464 | 3' | -57.4 | NC_005259.1 | + | 60551 | 0.75 | 0.232154 |
Target: 5'- gCUCGUCGGCAugcCCGGUgccGACGGUCa-- -3' miRNA: 3'- gGAGCAGCCGU---GGCCG---CUGCUAGagu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 67042 | 0.75 | 0.243996 |
Target: 5'- cCCUCGaCGGCGagcacCCGuGCGcCGAUCUCAa -3' miRNA: 3'- -GGAGCaGCCGU-----GGC-CGCuGCUAGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 67792 | 0.74 | 0.262694 |
Target: 5'- aCCUUGUUGGCACCGcguGCGAgcuugagccCGAUCUUg -3' miRNA: 3'- -GGAGCAGCCGUGGC---CGCU---------GCUAGAGu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 58735 | 0.73 | 0.303557 |
Target: 5'- -gUCGUUGGCACCGGCaccGGCGAagUUCGg -3' miRNA: 3'- ggAGCAGCCGUGGCCG---CUGCUa-GAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 58161 | 0.72 | 0.349135 |
Target: 5'- aCUCGUCGGCGCaCGGgaucuCGACGcacGUCUUg -3' miRNA: 3'- gGAGCAGCCGUG-GCC-----GCUGC---UAGAGu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 34868 | 0.72 | 0.35719 |
Target: 5'- uCCUCGUCGGUGCCGGacCGGgGugcCUCGg -3' miRNA: 3'- -GGAGCAGCCGUGGCC--GCUgCua-GAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 34050 | 0.71 | 0.408198 |
Target: 5'- uUCUUGUUGGCAUuguccgaccaCGGCGGCGGUgUCc -3' miRNA: 3'- -GGAGCAGCCGUG----------GCCGCUGCUAgAGu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 61562 | 0.71 | 0.417132 |
Target: 5'- gCCgacaCGGCACCGGCaguuGAgGAUCUCAu -3' miRNA: 3'- -GGagcaGCCGUGGCCG----CUgCUAGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 27376 | 0.71 | 0.426184 |
Target: 5'- gCC-CGgcaCGGUgACCGGCGGCGGUgUCGg -3' miRNA: 3'- -GGaGCa--GCCG-UGGCCGCUGCUAgAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 8316 | 0.71 | 0.435352 |
Target: 5'- gCCUUGUCGGagcuGCCGaCGGCGAUCa-- -3' miRNA: 3'- -GGAGCAGCCg---UGGCcGCUGCUAGagu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 43184 | 0.71 | 0.435352 |
Target: 5'- ---aGUCGGUACCgaccucGGCGGCGAUgUCGa -3' miRNA: 3'- ggagCAGCCGUGG------CCGCUGCUAgAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 68286 | 0.7 | 0.467349 |
Target: 5'- uCCUCGccccauacaucgggaUCgGGCGcguCCGGCGACGGgaUCUCGa -3' miRNA: 3'- -GGAGC---------------AG-CCGU---GGCCGCUGCU--AGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 51674 | 0.7 | 0.473122 |
Target: 5'- --gCGUCgGGCACgGGCGcgcgAUGGUCUCAu -3' miRNA: 3'- ggaGCAG-CCGUGgCCGC----UGCUAGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 61728 | 0.7 | 0.473122 |
Target: 5'- aCCUUGUCGaGCAggugugcguCCGGCGA-GAUCUg- -3' miRNA: 3'- -GGAGCAGC-CGU---------GGCCGCUgCUAGAgu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 21980 | 0.7 | 0.492615 |
Target: 5'- -gUCGUucuccccacCGGCACCGGCaagucGACGGUCaUCGg -3' miRNA: 3'- ggAGCA---------GCCGUGGCCG-----CUGCUAG-AGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 2239 | 0.69 | 0.5025 |
Target: 5'- aUUUGUCGGCGacagccgguaCGGCGACGAgUUCGa -3' miRNA: 3'- gGAGCAGCCGUg---------GCCGCUGCUaGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 34560 | 0.69 | 0.5025 |
Target: 5'- --cCGUCGGgGCCGaCGGCGAgguUCUCGg -3' miRNA: 3'- ggaGCAGCCgUGGCcGCUGCU---AGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 10320 | 0.69 | 0.5025 |
Target: 5'- -gUCGUCaucgGGC-UCGGCGACGggCUCGg -3' miRNA: 3'- ggAGCAG----CCGuGGCCGCUGCuaGAGU- -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 20486 | 0.69 | 0.522521 |
Target: 5'- aCCUCGacaccgccCGGCAgcucaUCGGCGACGGUgUCc -3' miRNA: 3'- -GGAGCa-------GCCGU-----GGCCGCUGCUAgAGu -5' |
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23464 | 3' | -57.4 | NC_005259.1 | + | 63675 | 0.69 | 0.532647 |
Target: 5'- aCCUCG--GGCACCGGCGcgccgGgGAUCUg- -3' miRNA: 3'- -GGAGCagCCGUGGCCGC-----UgCUAGAgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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