Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23470 | 5' | -57.3 | NC_005259.1 | + | 50697 | 0.67 | 0.582504 |
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Target: 5'- -cGCUGCUCGucgUCGCGCUUga---GCCg -3' miRNA: 3'- uuCGACGAGU---GGCGCGAGaagagCGG- -5' |
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| 23470 | 5' | -57.3 | NC_005259.1 | + | 52026 | 0.67 | 0.582504 |
|
Target: 5'- cAGGCcGCcgCACUGCGCgCUg-UCGCCg -3' miRNA: 3'- -UUCGaCGa-GUGGCGCGaGAagAGCGG- -5' |
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| 23470 | 5' | -57.3 | NC_005259.1 | + | 58823 | 0.68 | 0.495291 |
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Target: 5'- -cGCUGCUCggcacccucGCCGCcCUCggucucacccucgggCUCGCCg -3' miRNA: 3'- uuCGACGAG---------UGGCGcGAGaa-------------GAGCGG- -5' |
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| 23470 | 5' | -57.3 | NC_005259.1 | + | 59162 | 0.66 | 0.614922 |
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Target: 5'- gGAGCUGCUCAggGCGCUUauacauggcgCUUGUCu -3' miRNA: 3'- -UUCGACGAGUggCGCGAGaa--------GAGCGG- -5' |
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| 23470 | 5' | -57.3 | NC_005259.1 | + | 63646 | 0.67 | 0.571767 |
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Target: 5'- cGAGCUGCUCGCUG-GC-CgggaCGCCg -3' miRNA: 3'- -UUCGACGAGUGGCgCGaGaagaGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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