miRNA: 3'- uuCGaCGA-GUGGCGCGaGA--AG-AGCGG- -5'
miRNA: 3'- uUCGaCGaGUGGCGCGaga-AGAGCGG- -5'
miRNA: 3'- uuCgACGAGUGGCGCG--------------AGaa---GAGCGG- -5'
miRNA: 3'- -UUCGACGAGUGGCgCGagaaGAGCGG- -5'
miRNA: 3'- uuCGa--CGAGUGGCGCGAGaaGAgC-GG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23470 | 5' | -57.3 | NC_005259.1 | + | 31563 | 0.7 | 0.401668 |
|
Target: 5'- -uGCcGCUgCACCGCGC-CUgaUCgUCGCCa -3' miRNA: 3'- uuCGaCGA-GUGGCGCGaGA--AG-AGCGG- -5' |
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| 23470 | 5' | -57.3 | NC_005259.1 | + | 33129 | 0.71 | 0.366465 |
|
Target: 5'- cGGCaGC-CGCCGCGCgugaUCUUGCCg -3' miRNA: 3'- uUCGaCGaGUGGCGCGaga-AGAGCGG- -5' |
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| 23470 | 5' | -57.3 | NC_005259.1 | + | 13470 | 0.71 | 0.347208 |
|
Target: 5'- -uGgUGCUCACCGCGCugcccgacgaugucUCgguccCUCGCCc -3' miRNA: 3'- uuCgACGAGUGGCGCG--------------AGaa---GAGCGG- -5' |
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| 23470 | 5' | -57.3 | NC_005259.1 | + | 45550 | 0.71 | 0.333446 |
|
Target: 5'- cGGGUUGCUCGCCGgGCcaccCUUGCCc -3' miRNA: 3'- -UUCGACGAGUGGCgCGagaaGAGCGG- -5' |
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| 23470 | 5' | -57.3 | NC_005259.1 | + | 29143 | 0.74 | 0.2294 |
|
Target: 5'- -cGCcucGCUCACCGCGCUCggCUgGaCCg -3' miRNA: 3'- uuCGa--CGAGUGGCGCGAGaaGAgC-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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