Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23471 | 5' | -55.1 | NC_005259.1 | + | 50082 | 0.66 | 0.81955 |
Target: 5'- cCUGCGGCAgc-UCGcCGAUGgUGcGCCCg -3' miRNA: 3'- -GAUGCUGUaccAGU-GCUGCgAC-CGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 46563 | 0.66 | 0.81955 |
Target: 5'- -cGCGcCuAUGGUCGCGGCGUcGaccgcgccGCCCg -3' miRNA: 3'- gaUGCuG-UACCAGUGCUGCGaC--------CGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 32202 | 0.66 | 0.81955 |
Target: 5'- uCUGCGACuUGGccUCAgacacCGACGCcucGGCaCCg -3' miRNA: 3'- -GAUGCUGuACC--AGU-----GCUGCGa--CCG-GG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 6649 | 0.66 | 0.81955 |
Target: 5'- -gACGACAcGGUCGaggguguccgUGACGCgcgagcuGCCCg -3' miRNA: 3'- gaUGCUGUaCCAGU----------GCUGCGac-----CGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 22180 | 0.66 | 0.801097 |
Target: 5'- -cGCGcccauCGUGGUUGCGAccuugcagaCGUUGGCCa -3' miRNA: 3'- gaUGCu----GUACCAGUGCU---------GCGACCGGg -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 16793 | 0.66 | 0.791611 |
Target: 5'- --uCGACGcGGUCGCGuacaucGCGCUcggGGCCg -3' miRNA: 3'- gauGCUGUaCCAGUGC------UGCGA---CCGGg -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 9061 | 0.66 | 0.772173 |
Target: 5'- --cCGGCucgGUGGUCAUGGCcacaGCauaGGCCCg -3' miRNA: 3'- gauGCUG---UACCAGUGCUG----CGa--CCGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 39490 | 0.67 | 0.752182 |
Target: 5'- -cACGAgAuaucgagcuugaUGGcCGCGcCGCUGGCCa -3' miRNA: 3'- gaUGCUgU------------ACCaGUGCuGCGACCGGg -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 13462 | 0.67 | 0.752182 |
Target: 5'- -cGCGGCGcUGGugcUCACcGCGCUG-CCCg -3' miRNA: 3'- gaUGCUGU-ACC---AGUGcUGCGACcGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 49858 | 0.67 | 0.742005 |
Target: 5'- -gAgGAUcguUGGUCACGACGCguUGcGCCg -3' miRNA: 3'- gaUgCUGu--ACCAGUGCUGCG--AC-CGGg -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 21173 | 0.67 | 0.742005 |
Target: 5'- -gACGGCAgucgGGUCAuCGGCGC-GaGCaCCa -3' miRNA: 3'- gaUGCUGUa---CCAGU-GCUGCGaC-CG-GG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 65168 | 0.67 | 0.731722 |
Target: 5'- -cAUGGCccgccgGGUCAaGGCGCUGGUCg -3' miRNA: 3'- gaUGCUGua----CCAGUgCUGCGACCGGg -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 35672 | 0.67 | 0.731722 |
Target: 5'- -cGCgGugGUGGUgcacaGCGACGCcGuGCCCg -3' miRNA: 3'- gaUG-CugUACCAg----UGCUGCGaC-CGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 50942 | 0.67 | 0.729654 |
Target: 5'- -aACGGCucguugcauccuUGGcUCGCGugGCUGuCCCa -3' miRNA: 3'- gaUGCUGu-----------ACC-AGUGCugCGACcGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 51259 | 0.68 | 0.710881 |
Target: 5'- uUGCGGCucgcgGUGGUCACGguGCGC-GGCa- -3' miRNA: 3'- gAUGCUG-----UACCAGUGC--UGCGaCCGgg -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 5003 | 0.68 | 0.710881 |
Target: 5'- aCUACGGCAaggUGGUCGaGAuCGauccGGCCCc -3' miRNA: 3'- -GAUGCUGU---ACCAGUgCU-GCga--CCGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 37196 | 0.68 | 0.704567 |
Target: 5'- gCUGCGGCccuUGccGcCACcgccgccgccgccggGGCGCUGGCCCg -3' miRNA: 3'- -GAUGCUGu--AC--CaGUG---------------CUGCGACCGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 58570 | 0.68 | 0.700344 |
Target: 5'- gUugGGCcgGaUCGCGGCGCggcugccgGGCCg -3' miRNA: 3'- gAugCUGuaCcAGUGCUGCGa-------CCGGg -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 14060 | 0.68 | 0.668393 |
Target: 5'- -gGCGACGagaccagCACGGCGgUGGCCUa -3' miRNA: 3'- gaUGCUGUacca---GUGCUGCgACCGGG- -5' |
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23471 | 5' | -55.1 | NC_005259.1 | + | 55889 | 0.68 | 0.657664 |
Target: 5'- --cCGACcucgggGGUCGCGccgggguCGCUGGCCa -3' miRNA: 3'- gauGCUGua----CCAGUGCu------GCGACCGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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