Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23471 | 5' | -55.1 | NC_005259.1 | + | 29931 | 0.69 | 0.62538 |
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Target: 5'- -gACG-CGUGGcCGCcACGC-GGCCCg -3' miRNA: 3'- gaUGCuGUACCaGUGcUGCGaCCGGG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 57443 | 0.69 | 0.60387 |
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Target: 5'- -cGCGGCGaGGUUgguGCGACGCUgcggcaGGCCg -3' miRNA: 3'- gaUGCUGUaCCAG---UGCUGCGA------CCGGg -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 38881 | 0.7 | 0.540152 |
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Target: 5'- -aAUGGCgGUGuUgACGACGCUGGCCg -3' miRNA: 3'- gaUGCUG-UACcAgUGCUGCGACCGGg -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 65797 | 0.72 | 0.439943 |
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Target: 5'- cCU-CGACGcgcUGGUCGaaACGCUGGCCg -3' miRNA: 3'- -GAuGCUGU---ACCAGUgcUGCGACCGGg -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 56699 | 0.72 | 0.439943 |
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Target: 5'- gCUGCGAgGUGGcCACGAUGgUGuCCCc -3' miRNA: 3'- -GAUGCUgUACCaGUGCUGCgACcGGG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 42258 | 0.73 | 0.402895 |
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Target: 5'- gCUGUGAgGUGGUCACGuCGa-GGCCCu -3' miRNA: 3'- -GAUGCUgUACCAGUGCuGCgaCCGGG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 9824 | 0.78 | 0.214081 |
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Target: 5'- -cACcGCcgGGU-GCGGCGCUGGCCCa -3' miRNA: 3'- gaUGcUGuaCCAgUGCUGCGACCGGG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 24321 | 0.79 | 0.182503 |
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Target: 5'- --cCGACAUGGUC-CGgccucacGCGCUGGCCUa -3' miRNA: 3'- gauGCUGUACCAGuGC-------UGCGACCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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