Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23471 | 5' | -55.1 | NC_005259.1 | + | 21173 | 0.67 | 0.742005 |
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Target: 5'- -gACGGCAgucgGGUCAuCGGCGC-GaGCaCCa -3' miRNA: 3'- gaUGCUGUa---CCAGU-GCUGCGaC-CG-GG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 16793 | 0.66 | 0.791611 |
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Target: 5'- --uCGACGcGGUCGCGuacaucGCGCUcggGGCCg -3' miRNA: 3'- gauGCUGUaCCAGUGC------UGCGA---CCGGg -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 14060 | 0.68 | 0.668393 |
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Target: 5'- -gGCGACGagaccagCACGGCGgUGGCCUa -3' miRNA: 3'- gaUGCUGUacca---GUGCUGCgACCGGG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 13462 | 0.67 | 0.752182 |
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Target: 5'- -cGCGGCGcUGGugcUCACcGCGCUG-CCCg -3' miRNA: 3'- gaUGCUGU-ACC---AGUGcUGCGACcGGG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 9824 | 0.78 | 0.214081 |
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Target: 5'- -cACcGCcgGGU-GCGGCGCUGGCCCa -3' miRNA: 3'- gaUGcUGuaCCAgUGCUGCGACCGGG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 9061 | 0.66 | 0.772173 |
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Target: 5'- --cCGGCucgGUGGUCAUGGCcacaGCauaGGCCCg -3' miRNA: 3'- gauGCUG---UACCAGUGCUG----CGa--CCGGG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 6649 | 0.66 | 0.81955 |
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Target: 5'- -gACGACAcGGUCGaggguguccgUGACGCgcgagcuGCCCg -3' miRNA: 3'- gaUGCUGUaCCAGU----------GCUGCGac-----CGGG- -5' |
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| 23471 | 5' | -55.1 | NC_005259.1 | + | 5003 | 0.68 | 0.710881 |
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Target: 5'- aCUACGGCAaggUGGUCGaGAuCGauccGGCCCc -3' miRNA: 3'- -GAUGCUGU---ACCAGUgCU-GCga--CCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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