Results 1 - 20 of 78 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23475 | 5' | -59.3 | NC_005259.1 | + | 68741 | 0.69 | 0.38795 |
Target: 5'- cGCUUGCCGAGCUCgaccccgGCGaccucGCCaUCGGUg -3' miRNA: 3'- -UGAGCGGCUUGAG-------CGCa----CGG-AGCCGg -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 67354 | 0.7 | 0.358742 |
Target: 5'- cGCUCGCCGAcCUCGCGccgGaucuuggggaaauuaCgCUUGGCCu -3' miRNA: 3'- -UGAGCGGCUuGAGCGCa--C---------------G-GAGCCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 65985 | 0.66 | 0.550368 |
Target: 5'- gGCgCGCCGAgcagccGCUCGaCGUGgugcgcacCCUCggGGCCg -3' miRNA: 3'- -UGaGCGGCU------UGAGC-GCAC--------GGAG--CCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 64785 | 0.78 | 0.114676 |
Target: 5'- gGCcCGCUGcGACUggcUGUGUGCCUCGGCCu -3' miRNA: 3'- -UGaGCGGC-UUGA---GCGCACGGAGCCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 64307 | 0.73 | 0.243006 |
Target: 5'- aGCUCGCCGAuGCUCuCGgaaaagucccaUGCCUCGGaCUg -3' miRNA: 3'- -UGAGCGGCU-UGAGcGC-----------ACGGAGCC-GG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 62024 | 0.67 | 0.519925 |
Target: 5'- cGCUCGCgCuguGugUCGaCGUaGuCCUCGGCCc -3' miRNA: 3'- -UGAGCG-Gc--UugAGC-GCA-C-GGAGCCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 61729 | 0.67 | 0.490164 |
Target: 5'- cCUUGUCGAGCagGUGUGCgUcCGGCg -3' miRNA: 3'- uGAGCGGCUUGagCGCACGgA-GCCGg -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 60550 | 0.66 | 0.570967 |
Target: 5'- cGCUCGUCGGcauGCcCG-GUGCCgacggucaggCGGCCc -3' miRNA: 3'- -UGAGCGGCU---UGaGCgCACGGa---------GCCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 60502 | 0.66 | 0.602187 |
Target: 5'- cACgCGCCGGugUUGuCGUagugcGCCaUGGCCg -3' miRNA: 3'- -UGaGCGGCUugAGC-GCA-----CGGaGCCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 60235 | 0.72 | 0.274611 |
Target: 5'- aGCUCGCUGGcacGCUUGCGUGCgggugcaaggCU-GGCCa -3' miRNA: 3'- -UGAGCGGCU---UGAGCGCACG----------GAgCCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 60134 | 0.68 | 0.433207 |
Target: 5'- cCUCGUCcug--CGCG-GCCUUGGCCg -3' miRNA: 3'- uGAGCGGcuugaGCGCaCGGAGCCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 58912 | 0.71 | 0.291611 |
Target: 5'- gGCUCGUCGGcggucuugucgucgGCgggCGCGguggcagcgucgGCCUUGGCCa -3' miRNA: 3'- -UGAGCGGCU--------------UGa--GCGCa-----------CGGAGCCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 58862 | 0.77 | 0.117789 |
Target: 5'- gGCUCGCCGucgUCGuCGUcGCCUCGGCa -3' miRNA: 3'- -UGAGCGGCuugAGC-GCA-CGGAGCCGg -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 58809 | 0.75 | 0.184102 |
Target: 5'- cCUCGgCGGGCUCGCGcUGC-UCGGCa -3' miRNA: 3'- uGAGCgGCUUGAGCGC-ACGgAGCCGg -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 58205 | 0.68 | 0.442437 |
Target: 5'- gGCUUGCCGGugUCaGgGUcagugGCCUCGaGCUc -3' miRNA: 3'- -UGAGCGGCUugAG-CgCA-----CGGAGC-CGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 57708 | 0.68 | 0.442437 |
Target: 5'- gGCUUGCCcGACUCGCGgGUCU--GCCa -3' miRNA: 3'- -UGAGCGGcUUGAGCGCaCGGAgcCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 57528 | 0.69 | 0.424091 |
Target: 5'- cGCUUGCCGAcgGCgaugGCGU-CCUCGGUg -3' miRNA: 3'- -UGAGCGGCU--UGag--CGCAcGGAGCCGg -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 56909 | 0.69 | 0.406208 |
Target: 5'- gGC-CGCCGcAGCagGCGUGCCcgcgUCGGUg -3' miRNA: 3'- -UGaGCGGC-UUGagCGCACGG----AGCCGg -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 56417 | 0.67 | 0.5 |
Target: 5'- gGCcCGCCGAGCUCaagcagagcaGCGagcGCCg-GGCCa -3' miRNA: 3'- -UGaGCGGCUUGAG----------CGCa--CGGagCCGG- -5' |
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23475 | 5' | -59.3 | NC_005259.1 | + | 55869 | 0.69 | 0.380291 |
Target: 5'- aACUCGCCGAGCgccUCGCcccGaCCUCGGg- -3' miRNA: 3'- -UGAGCGGCUUG---AGCGca-C-GGAGCCgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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