Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2350 | 3' | -59.2 | NC_001416.1 | + | 16889 | 0.66 | 0.445381 |
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Target: 5'- uCAGcCACAGCccGGCGCGgugugccugCcCCCGGCUg -3' miRNA: 3'- uGUC-GUGUCG--UCGUGUa--------GcGGGCCGA- -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 16998 | 0.69 | 0.276565 |
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Target: 5'- cCGGCAUAgucaucaucGCAGauuucaauaACAUCGCCCGGUa -3' miRNA: 3'- uGUCGUGU---------CGUCg--------UGUAGCGGGCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 17471 | 0.67 | 0.36246 |
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Target: 5'- gACGGCAgugAGCGGCugGUCagcacgGCCCGGaCg -3' miRNA: 3'- -UGUCGUg--UCGUCGugUAG------CGGGCC-Ga -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 17629 | 0.66 | 0.426035 |
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Target: 5'- gGCAGCACcGUcgaggauugAGCugA-CGCCgGGCUa -3' miRNA: 3'- -UGUCGUGuCG---------UCGugUaGCGGgCCGA- -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 20080 | 0.67 | 0.37994 |
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Target: 5'- uCAGCACGcgccGcCAGCACGUCcGCCggacaGGCUg -3' miRNA: 3'- uGUCGUGU----C-GUCGUGUAG-CGGg----CCGA- -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 20094 | 0.66 | 0.445381 |
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Target: 5'- aGCAGCugAuGCAcugGCAUCGCC-GGCUg -3' miRNA: 3'- -UGUCGugU-CGUcg-UGUAGCGGgCCGA- -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 21725 | 0.67 | 0.37994 |
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Target: 5'- gACAGuCAgGGCAGCcacagucacuCAUUGUCCGGUa -3' miRNA: 3'- -UGUC-GUgUCGUCGu---------GUAGCGGGCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 22451 | 0.66 | 0.435646 |
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Target: 5'- uGCAGCAUccugaAGCGGCGCAauaUGCUCacuGGCUa -3' miRNA: 3'- -UGUCGUG-----UCGUCGUGUa--GCGGG---CCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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