Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2350 | 3' | -59.2 | NC_001416.1 | + | 12914 | 0.66 | 0.407196 |
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Target: 5'- -uGGCAUAGCcGCGCAUCaG-CCGGUa -3' miRNA: 3'- ugUCGUGUCGuCGUGUAG-CgGGCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 2079 | 0.66 | 0.41655 |
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Target: 5'- cACA-CGCAGCAGCGucuguuCAUCGUCgUGGCg -3' miRNA: 3'- -UGUcGUGUCGUCGU------GUAGCGG-GCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 12004 | 0.66 | 0.42508 |
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Target: 5'- aGCAGgGCAGCaugAGCACugucuuccugacgAUCGCCCG-Ca -3' miRNA: 3'- -UGUCgUGUCG---UCGUG-------------UAGCGGGCcGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 17629 | 0.66 | 0.426035 |
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Target: 5'- gGCAGCACcGUcgaggauugAGCugA-CGCCgGGCUa -3' miRNA: 3'- -UGUCGUGuCG---------UCGugUaGCGGgCCGA- -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 4783 | 0.66 | 0.435646 |
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Target: 5'- gACGGCAUucuGCucgauauGgACA-CGCCCGGCg -3' miRNA: 3'- -UGUCGUGu--CGu------CgUGUaGCGGGCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 22451 | 0.66 | 0.435646 |
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Target: 5'- uGCAGCAUccugaAGCGGCGCAauaUGCUCacuGGCUa -3' miRNA: 3'- -UGUCGUG-----UCGUCGUGUa--GCGGG---CCGA- -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 20094 | 0.66 | 0.445381 |
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Target: 5'- aGCAGCugAuGCAcugGCAUCGCC-GGCUg -3' miRNA: 3'- -UGUCGugU-CGUcg-UGUAGCGGgCCGA- -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 16889 | 0.66 | 0.445381 |
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Target: 5'- uCAGcCACAGCccGGCGCGgugugccugCcCCCGGCUg -3' miRNA: 3'- uGUC-GUGUCG--UCGUGUa--------GcGGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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