Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2350 | 3' | -59.2 | NC_001416.1 | + | 8575 | 0.66 | 0.397975 |
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Target: 5'- uCAGUugauGCuGCAGCGCAUaGCCCaGCUc -3' miRNA: 3'- uGUCG----UGuCGUCGUGUAgCGGGcCGA- -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 3455 | 0.68 | 0.321254 |
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Target: 5'- cCGGCACGGCAGUuccgGCuGUCG-CCGGUa -3' miRNA: 3'- uGUCGUGUCGUCG----UG-UAGCgGGCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 14488 | 0.68 | 0.305781 |
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Target: 5'- uGCGGCACA-CAGCGgaacuuaugaauCGUCcCCCGGCa -3' miRNA: 3'- -UGUCGUGUcGUCGU------------GUAGcGGGCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 10338 | 0.69 | 0.283654 |
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Target: 5'- -gAGCACGGU-GUACGUCaGCCCGGa- -3' miRNA: 3'- ugUCGUGUCGuCGUGUAG-CGGGCCga -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 16998 | 0.69 | 0.276565 |
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Target: 5'- cCGGCAUAgucaucaucGCAGauuucaauaACAUCGCCCGGUa -3' miRNA: 3'- uGUCGUGU---------CGUCg--------UGUAGCGGGCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 16586 | 0.7 | 0.243233 |
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Target: 5'- cCGGCAUA-CAGCGCAUCGCCa-GCa -3' miRNA: 3'- uGUCGUGUcGUCGUGUAGCGGgcCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 14765 | 0.7 | 0.243233 |
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Target: 5'- uAUGGUGCAGCAGCucgcCGUCGCCgcaguaaauugCGGCg -3' miRNA: 3'- -UGUCGUGUCGUCGu---GUAGCGG-----------GCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 16889 | 0.66 | 0.445381 |
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Target: 5'- uCAGcCACAGCccGGCGCGgugugccugCcCCCGGCUg -3' miRNA: 3'- uGUC-GUGUCG--UCGUGUa--------GcGGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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