Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23501 | 5' | -58.2 | NC_005259.1 | + | 3325 | 0.66 | 0.604502 |
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Target: 5'- aCGUGAGcGCCGGGCagcucugcgUGcUCGGCacgaCUCGAc -3' miRNA: 3'- -GCGCUC-CGGUUCG---------ACaAGCCG----GAGCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 62671 | 0.66 | 0.604502 |
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Target: 5'- aGCGAGGCCAcGacc-UCGuCCUCGAg -3' miRNA: 3'- gCGCUCCGGUuCgacaAGCcGGAGCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 34001 | 0.66 | 0.615123 |
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Target: 5'- gGUGAGcuGCaaccGGCUGUaucCGGCCUCGGc -3' miRNA: 3'- gCGCUC--CGgu--UCGACAa--GCCGGAGCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 12054 | 0.66 | 0.625758 |
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Target: 5'- -cCGAGGCCGAGCUcugCGGCagacacCGAg -3' miRNA: 3'- gcGCUCCGGUUCGAcaaGCCGga----GCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 29048 | 0.66 | 0.625758 |
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Target: 5'- aCGaCGAGaGCCGcgAGCgcgGUaUCGGCC-CGAu -3' miRNA: 3'- -GC-GCUC-CGGU--UCGa--CA-AGCCGGaGCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 36219 | 0.66 | 0.625758 |
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Target: 5'- gCGCGAuGCCGAGCaccUUGGCCagcgCGAg -3' miRNA: 3'- -GCGCUcCGGUUCGacaAGCCGGa---GCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 6548 | 0.66 | 0.636398 |
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Target: 5'- gGCGAgcGGUgAAGCUGUgcgCGgcgugucaggccGCCUCGGa -3' miRNA: 3'- gCGCU--CCGgUUCGACAa--GC------------CGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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