Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23501 | 5' | -58.2 | NC_005259.1 | + | 17808 | 0.7 | 0.362447 |
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Target: 5'- aGCGAGGCCGgugAGCUcagcgaGGCCaUCGAc -3' miRNA: 3'- gCGCUCCGGU---UCGAcaag--CCGG-AGCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 1786 | 0.7 | 0.396692 |
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Target: 5'- cCGaCGAGGCCcccaAGGCUGUcaUGGCCgacgCGAg -3' miRNA: 3'- -GC-GCUCCGG----UUCGACAa-GCCGGa---GCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 28135 | 0.7 | 0.405573 |
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Target: 5'- cCGaCGuAGGCgauGAGCUGccCGGCCUCGAc -3' miRNA: 3'- -GC-GC-UCCGg--UUCGACaaGCCGGAGCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 62834 | 0.69 | 0.411863 |
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Target: 5'- cCGCGAGGUCGAGCgcgcgGUugacgagcagccgcUCGGCg-CGAg -3' miRNA: 3'- -GCGCUCCGGUUCGa----CA--------------AGCCGgaGCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 10630 | 0.69 | 0.43295 |
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Target: 5'- gCGuCGAGGCCGaacAGCU---UGGCCUCGc -3' miRNA: 3'- -GC-GCUCCGGU---UCGAcaaGCCGGAGCu -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 23059 | 0.69 | 0.455609 |
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Target: 5'- gGCGAGGgaagcgacccgacacCCAAGCUcGUUCGGCagggaccggUCGAg -3' miRNA: 3'- gCGCUCC---------------GGUUCGA-CAAGCCGg--------AGCU- -5' |
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| 23501 | 5' | -58.2 | NC_005259.1 | + | 6548 | 0.66 | 0.636398 |
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Target: 5'- gGCGAgcGGUgAAGCUGUgcgCGgcgugucaggccGCCUCGGa -3' miRNA: 3'- gCGCU--CCGgUUCGACAa--GC------------CGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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