miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23501 5' -58.2 NC_005259.1 + 1786 0.7 0.396692
Target:  5'- cCGaCGAGGCCcccaAGGCUGUcaUGGCCgacgCGAg -3'
miRNA:   3'- -GC-GCUCCGG----UUCGACAa-GCCGGa---GCU- -5'
23501 5' -58.2 NC_005259.1 + 17808 0.7 0.362447
Target:  5'- aGCGAGGCCGgugAGCUcagcgaGGCCaUCGAc -3'
miRNA:   3'- gCGCUCCGGU---UCGAcaag--CCGG-AGCU- -5'
23501 5' -58.2 NC_005259.1 + 64765 0.71 0.327987
Target:  5'- uCGCGAcggugcgccucggcGGCCcgcugcgacuGGCUGUgugccUCGGCCUCGGc -3'
miRNA:   3'- -GCGCU--------------CCGGu---------UCGACA-----AGCCGGAGCU- -5'
23501 5' -58.2 NC_005259.1 + 16599 0.72 0.307618
Target:  5'- cCGCGAGGCCAucGGaCUGUUCaacgGGCCg--- -3'
miRNA:   3'- -GCGCUCCGGU--UC-GACAAG----CCGGagcu -5'
23501 5' -58.2 NC_005259.1 + 9534 0.73 0.272483
Target:  5'- cCGCcGGGUCAccggagAGCUGccCGGCCUCGAc -3'
miRNA:   3'- -GCGcUCCGGU------UCGACaaGCCGGAGCU- -5'
23501 5' -58.2 NC_005259.1 + 46294 0.73 0.252991
Target:  5'- aGCGGGGCCGccguGGCUGcguuguucgCGGCCcCGAg -3'
miRNA:   3'- gCGCUCCGGU----UCGACaa-------GCCGGaGCU- -5'
23501 5' -58.2 NC_005259.1 + 47645 0.74 0.232302
Target:  5'- aGCGAGGCCcucuuugaacguGCUGUUgaUGGCCUUGGc -3'
miRNA:   3'- gCGCUCCGGuu----------CGACAA--GCCGGAGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.