Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23501 | 5' | -58.2 | NC_005259.1 | + | 1786 | 0.7 | 0.396692 |
Target: 5'- cCGaCGAGGCCcccaAGGCUGUcaUGGCCgacgCGAg -3' miRNA: 3'- -GC-GCUCCGG----UUCGACAa-GCCGGa---GCU- -5' |
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23501 | 5' | -58.2 | NC_005259.1 | + | 17808 | 0.7 | 0.362447 |
Target: 5'- aGCGAGGCCGgugAGCUcagcgaGGCCaUCGAc -3' miRNA: 3'- gCGCUCCGGU---UCGAcaag--CCGG-AGCU- -5' |
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23501 | 5' | -58.2 | NC_005259.1 | + | 64765 | 0.71 | 0.327987 |
Target: 5'- uCGCGAcggugcgccucggcGGCCcgcugcgacuGGCUGUgugccUCGGCCUCGGc -3' miRNA: 3'- -GCGCU--------------CCGGu---------UCGACA-----AGCCGGAGCU- -5' |
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23501 | 5' | -58.2 | NC_005259.1 | + | 16599 | 0.72 | 0.307618 |
Target: 5'- cCGCGAGGCCAucGGaCUGUUCaacgGGCCg--- -3' miRNA: 3'- -GCGCUCCGGU--UC-GACAAG----CCGGagcu -5' |
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23501 | 5' | -58.2 | NC_005259.1 | + | 9534 | 0.73 | 0.272483 |
Target: 5'- cCGCcGGGUCAccggagAGCUGccCGGCCUCGAc -3' miRNA: 3'- -GCGcUCCGGU------UCGACaaGCCGGAGCU- -5' |
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23501 | 5' | -58.2 | NC_005259.1 | + | 46294 | 0.73 | 0.252991 |
Target: 5'- aGCGGGGCCGccguGGCUGcguuguucgCGGCCcCGAg -3' miRNA: 3'- gCGCUCCGGU----UCGACaa-------GCCGGaGCU- -5' |
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23501 | 5' | -58.2 | NC_005259.1 | + | 47645 | 0.74 | 0.232302 |
Target: 5'- aGCGAGGCCcucuuugaacguGCUGUUgaUGGCCUUGGc -3' miRNA: 3'- gCGCUCCGGuu----------CGACAA--GCCGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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