miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23501 5' -58.2 NC_005259.1 + 51774 0.66 0.593903
Target:  5'- gGCGAGcGCCuuGAGgUaUUCGGCgCUCGGc -3'
miRNA:   3'- gCGCUC-CGG--UUCgAcAAGCCG-GAGCU- -5'
23501 5' -58.2 NC_005259.1 + 52265 0.67 0.572804
Target:  5'- aGgGGGGCCGGGCagacagGacCGGCC-CGAu -3'
miRNA:   3'- gCgCUCCGGUUCGa-----CaaGCCGGaGCU- -5'
23501 5' -58.2 NC_005259.1 + 56380 0.67 0.572804
Target:  5'- uCGCG-GGCCGcgucGGCUGaUCG-UCUCGAu -3'
miRNA:   3'- -GCGCuCCGGU----UCGACaAGCcGGAGCU- -5'
23501 5' -58.2 NC_005259.1 + 62671 0.66 0.604502
Target:  5'- aGCGAGGCCAcGacc-UCGuCCUCGAg -3'
miRNA:   3'- gCGCUCCGGUuCgacaAGCcGGAGCU- -5'
23501 5' -58.2 NC_005259.1 + 62834 0.69 0.411863
Target:  5'- cCGCGAGGUCGAGCgcgcgGUugacgagcagccgcUCGGCg-CGAg -3'
miRNA:   3'- -GCGCUCCGGUUCGa----CA--------------AGCCGgaGCU- -5'
23501 5' -58.2 NC_005259.1 + 64765 0.71 0.327987
Target:  5'- uCGCGAcggugcgccucggcGGCCcgcugcgacuGGCUGUgugccUCGGCCUCGGc -3'
miRNA:   3'- -GCGCU--------------CCGGu---------UCGACA-----AGCCGGAGCU- -5'
23501 5' -58.2 NC_005259.1 + 67916 0.68 0.48086
Target:  5'- gGCGAGGUCAAGCgcguaGGUCgCGAg -3'
miRNA:   3'- gCGCUCCGGUUCGacaagCCGGaGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.