Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23502 | 5' | -54.4 | NC_005259.1 | + | 59125 | 0.7 | 0.559915 |
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Target: 5'- -gCAUCGAGCAgAgCGUCGaGCUCGGc -3' miRNA: 3'- gaGUAGUUCGUgUgGCGGCaCGAGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 24570 | 0.7 | 0.538466 |
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Target: 5'- aCUCGU---GgGCACCGCaCGUGCUCAa -3' miRNA: 3'- -GAGUAguuCgUGUGGCG-GCACGAGUc -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 56653 | 0.71 | 0.527847 |
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Target: 5'- -gCAUCGAGgGCACCGCgCGUcacggGCUCGu -3' miRNA: 3'- gaGUAGUUCgUGUGGCG-GCA-----CGAGUc -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 12316 | 0.72 | 0.436499 |
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Target: 5'- --uGUCGGuGCGCACCGCCGccaGCUCAc -3' miRNA: 3'- gagUAGUU-CGUGUGGCGGCa--CGAGUc -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 15875 | 0.72 | 0.435534 |
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Target: 5'- uCUCAUCGGGCACACCGaaGagucgacUGCgCAGa -3' miRNA: 3'- -GAGUAGUUCGUGUGGCggC-------ACGaGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 50402 | 0.73 | 0.417444 |
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Target: 5'- gCUCGcCGAGCGCGCCGgugccuugaCCG-GCUCGGg -3' miRNA: 3'- -GAGUaGUUCGUGUGGC---------GGCaCGAGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 1406 | 0.74 | 0.372107 |
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Target: 5'- uCUCAUacgaccCGAGCACGgcauaCGUCGUGCUCGGg -3' miRNA: 3'- -GAGUA------GUUCGUGUg----GCGGCACGAGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 26206 | 0.74 | 0.338328 |
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Target: 5'- -gCAU--GGC-CGCCGCCGUGUUCAGg -3' miRNA: 3'- gaGUAguUCGuGUGGCGGCACGAGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 48597 | 0.76 | 0.270001 |
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Target: 5'- gCUUGUCGAGCGCgggcgcuACCGCCGUGCgCAc -3' miRNA: 3'- -GAGUAGUUCGUG-------UGGCGGCACGaGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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