Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23502 | 5' | -54.4 | NC_005259.1 | + | 47280 | 0.69 | 0.603441 |
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Target: 5'- -gCAcCGAGCAgACCGCCGUcGC-CGGa -3' miRNA: 3'- gaGUaGUUCGUgUGGCGGCA-CGaGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 48597 | 0.76 | 0.270001 |
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Target: 5'- gCUUGUCGAGCGCgggcgcuACCGCCGUGCgCAc -3' miRNA: 3'- -GAGUAGUUCGUG-------UGGCGGCACGaGUc -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 50402 | 0.73 | 0.417444 |
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Target: 5'- gCUCGcCGAGCGCGCCGgugccuugaCCG-GCUCGGg -3' miRNA: 3'- -GAGUaGUUCGUGUGGC---------GGCaCGAGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 56653 | 0.71 | 0.527847 |
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Target: 5'- -gCAUCGAGgGCACCGCgCGUcacggGCUCGu -3' miRNA: 3'- gaGUAGUUCgUGUGGCG-GCA-----CGAGUc -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 57626 | 0.66 | 0.813761 |
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Target: 5'- gCUCAUCGAGCggaaccauguGC-CCGCCGgGUaCAGc -3' miRNA: 3'- -GAGUAGUUCG----------UGuGGCGGCaCGaGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 59125 | 0.7 | 0.559915 |
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Target: 5'- -gCAUCGAGCAgAgCGUCGaGCUCGGc -3' miRNA: 3'- gaGUAGUUCGUgUgGCGGCaCGAGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 60134 | 0.67 | 0.743239 |
|
Target: 5'- cCUCGUCcuGCGCggccuugGCCGCCGc-CUCAGc -3' miRNA: 3'- -GAGUAGuuCGUG-------UGGCGGCacGAGUC- -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 62376 | 0.68 | 0.676903 |
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Target: 5'- gCUCAUCGAGUagcagauccaaaccGC-CCGCC-UGCUCGa -3' miRNA: 3'- -GAGUAGUUCG--------------UGuGGCGGcACGAGUc -5' |
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| 23502 | 5' | -54.4 | NC_005259.1 | + | 66178 | 0.68 | 0.65832 |
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Target: 5'- gCUCAUC-GGCAcCGCUGCCgGUGCaguacgggUCAGg -3' miRNA: 3'- -GAGUAGuUCGU-GUGGCGG-CACG--------AGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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