Results 1 - 20 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23508 | 3' | -56.3 | NC_005259.1 | + | 17759 | 0.75 | 0.245951 |
Target: 5'- uGAGGCCGCCGcGCGcCGGGCcgagCGCAc -3' miRNA: 3'- gCUCUGGUGGC-CGUuGCUCGua--GCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 54151 | 0.74 | 0.271896 |
Target: 5'- uCGAGGCC--CGGCGAUGGGCAUacCGCAa -3' miRNA: 3'- -GCUCUGGugGCCGUUGCUCGUA--GCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 25745 | 0.74 | 0.276657 |
Target: 5'- aGGGACCGCCGcGCAcacuauaccgcuauGCGGGCAgcucUCGCc -3' miRNA: 3'- gCUCUGGUGGC-CGU--------------UGCUCGU----AGCGu -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 6117 | 0.73 | 0.330287 |
Target: 5'- cCGAGGCCGCC-GCuacGCGGGCAcUGCAa -3' miRNA: 3'- -GCUCUGGUGGcCGu--UGCUCGUaGCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 16511 | 0.73 | 0.354448 |
Target: 5'- cCGAGAgCGa-GGCGcuGCGAGCGUCGCu -3' miRNA: 3'- -GCUCUgGUggCCGU--UGCUCGUAGCGu -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 67984 | 0.72 | 0.37983 |
Target: 5'- gCGuGGCCACC-GCcGCGAGCGUCGa- -3' miRNA: 3'- -GCuCUGGUGGcCGuUGCUCGUAGCgu -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 27159 | 0.71 | 0.415512 |
Target: 5'- uGGGGCCAacgaugccgUCGGCGAUGAGCAggcUGCGa -3' miRNA: 3'- gCUCUGGU---------GGCCGUUGCUCGUa--GCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 37273 | 0.71 | 0.415512 |
Target: 5'- uGAGGCCGcCCGGCAggACuuGCAUgGCGg -3' miRNA: 3'- gCUCUGGU-GGCCGU--UGcuCGUAgCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 40042 | 0.71 | 0.434106 |
Target: 5'- uGAGGUCGCCGGUGAuCGGGUcgGUCGCGa -3' miRNA: 3'- gCUCUGGUGGCCGUU-GCUCG--UAGCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 13172 | 0.7 | 0.462878 |
Target: 5'- cCGAGACCGcCCGGCAGCucGCcaUGCu -3' miRNA: 3'- -GCUCUGGU-GGCCGUUGcuCGuaGCGu -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 16601 | 0.7 | 0.482604 |
Target: 5'- gCGAGGCCAUCGGacuguuCAACGGGcCGagGCAg -3' miRNA: 3'- -GCUCUGGUGGCC------GUUGCUC-GUagCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 63555 | 0.7 | 0.482604 |
Target: 5'- uCGGcGGCCACCGacGCGACGAGCGggauauaCGCc -3' miRNA: 3'- -GCU-CUGGUGGC--CGUUGCUCGUa------GCGu -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 36045 | 0.7 | 0.486599 |
Target: 5'- gCGAGGCCACCcGCG-CGAGCAccaccugcugugugcUCGUc -3' miRNA: 3'- -GCUCUGGUGGcCGUuGCUCGU---------------AGCGu -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 23363 | 0.7 | 0.492619 |
Target: 5'- gCGAGGCCGCCgaGGCAugGAuCGUCa-- -3' miRNA: 3'- -GCUCUGGUGG--CCGUugCUcGUAGcgu -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 32361 | 0.7 | 0.492619 |
Target: 5'- uGAuGACCGCCGuGUAggGCGGGCAcugcccgccgUCGCGa -3' miRNA: 3'- gCU-CUGGUGGC-CGU--UGCUCGU----------AGCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 46295 | 0.7 | 0.499686 |
Target: 5'- gCGGGGCCGCCGuggcugcguuguucGCGGCcccGAGgGUCGCGu -3' miRNA: 3'- -GCUCUGGUGGC--------------CGUUG---CUCgUAGCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 53667 | 0.7 | 0.502728 |
Target: 5'- cCGAGGCC-CCac--GCGGGCAUCGCGa -3' miRNA: 3'- -GCUCUGGuGGccguUGCUCGUAGCGU- -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 45740 | 0.69 | 0.512927 |
Target: 5'- cCGAGGCCGCgcaccgccuCGGgGAUGAGCA-CGCc -3' miRNA: 3'- -GCUCUGGUG---------GCCgUUGCUCGUaGCGu -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 52575 | 0.69 | 0.523209 |
Target: 5'- --cGACCggACCGGCc-UGAGCGUCGCc -3' miRNA: 3'- gcuCUGG--UGGCCGuuGCUCGUAGCGu -5' |
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23508 | 3' | -56.3 | NC_005259.1 | + | 56656 | 0.69 | 0.53357 |
Target: 5'- uCGAGGgCACCGcGCGucACGGGCucGUUGCGc -3' miRNA: 3'- -GCUCUgGUGGC-CGU--UGCUCG--UAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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