Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23508 | 5' | -64.5 | NC_005259.1 | + | 2203 | 0.66 | 0.355642 |
Target: 5'- cGGCaAGGUGcugcgcaccgagcGCCCGGcCUCGCCauuugucGGCg -3' miRNA: 3'- -UCGcUCCAC-------------CGGGCC-GAGCGGcu-----CCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 8431 | 0.66 | 0.348625 |
Target: 5'- uGCGAugcucguugccGGUGGUCuCGGUgugGUCGAGGUa -3' miRNA: 3'- uCGCU-----------CCACCGG-GCCGag-CGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 36412 | 0.66 | 0.348625 |
Target: 5'- gAGCGccucaaGGGUcauGGUCUGcGCagcgUCGCCGAGGUc -3' miRNA: 3'- -UCGC------UCCA---CCGGGC-CG----AGCGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 47057 | 0.66 | 0.347852 |
Target: 5'- gAGCGuguuGGUGaGCgaGGCgagCGCCGAgacgaugGGCa -3' miRNA: 3'- -UCGCu---CCAC-CGggCCGa--GCGGCU-------CCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 68844 | 0.66 | 0.345539 |
Target: 5'- cGGUGAGGUGGgCCaucaugucgacuuGCccgCGCCGGGcGCg -3' miRNA: 3'- -UCGCUCCACCgGGc------------CGa--GCGGCUC-CG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 35656 | 0.66 | 0.341708 |
Target: 5'- uGGCGAgGGUGGCCagcgCGGUgguggugcacagcgaCGCCGugcccGGCg -3' miRNA: 3'- -UCGCU-CCACCGG----GCCGa--------------GCGGCu----CCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 42917 | 0.66 | 0.340946 |
Target: 5'- cGUu-GGUGGCCUugagcaugaGGCccUCGCCGuGGGCg -3' miRNA: 3'- uCGcuCCACCGGG---------CCG--AGCGGC-UCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 48051 | 0.66 | 0.340946 |
Target: 5'- ----cGGUGGCCaCGGCggguagCGCCGccGCg -3' miRNA: 3'- ucgcuCCACCGG-GCCGa-----GCGGCucCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 6209 | 0.66 | 0.340185 |
Target: 5'- gAGCGGGGUGaGCC--GCcgCGCCGAcaccgugaccgugGGCg -3' miRNA: 3'- -UCGCUCCAC-CGGgcCGa-GCGGCU-------------CCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 63658 | 0.66 | 0.326694 |
Target: 5'- uGGCcGGGacgccggacaccucgGGCaCCGGCgCGCCGGGGa -3' miRNA: 3'- -UCGcUCCa--------------CCG-GGCCGaGCGGCUCCg -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 60053 | 0.66 | 0.32522 |
Target: 5'- -uCGGGGUcuucucgggcucaGGCUcgggCGGCUCGCCGAcuuucucgauGGCg -3' miRNA: 3'- ucGCUCCA-------------CCGG----GCCGAGCGGCU----------CCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 46676 | 0.66 | 0.318648 |
Target: 5'- cGGCGAGcucGGCCUGaGC-CGCCGccGCg -3' miRNA: 3'- -UCGCUCca-CCGGGC-CGaGCGGCucCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 42260 | 0.66 | 0.311462 |
Target: 5'- uGUGAGGUGGUCa----CGUCGAGGCc -3' miRNA: 3'- uCGCUCCACCGGgccgaGCGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 56405 | 0.66 | 0.311462 |
Target: 5'- -uCGAuGGU-GCCCGGCcCGCCGAGcucaaGCa -3' miRNA: 3'- ucGCU-CCAcCGGGCCGaGCGGCUC-----CG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 23287 | 0.66 | 0.310751 |
Target: 5'- gGGCGcGGUGGUuggguaucgggguCCGGCgcggucggugUCGCCGAGcCg -3' miRNA: 3'- -UCGCuCCACCG-------------GGCCG----------AGCGGCUCcG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 28241 | 0.67 | 0.30721 |
Target: 5'- cGGCGGGGUGGucgaucacgauCCauugcccgaaaccguCGGCUCuGCCGAuguaGGCc -3' miRNA: 3'- -UCGCUCCACC-----------GG---------------GCCGAG-CGGCU----CCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 45488 | 0.67 | 0.3044 |
Target: 5'- uGGCcgcuGGUGGCCUuGgUCGCUGuGGCc -3' miRNA: 3'- -UCGcu--CCACCGGGcCgAGCGGCuCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 27992 | 0.67 | 0.297463 |
Target: 5'- ---uGGGUGGUgCGGg-UGCCGGGGCg -3' miRNA: 3'- ucgcUCCACCGgGCCgaGCGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 26807 | 0.67 | 0.297463 |
Target: 5'- cGCccGGuUGGCcgguaCCGGCgguggUGCCGGGGCg -3' miRNA: 3'- uCGcuCC-ACCG-----GGCCGa----GCGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 27476 | 0.67 | 0.29609 |
Target: 5'- gGGcCGGGGUcGGUgUGGCcaccgccccacgCGCCGAGGUc -3' miRNA: 3'- -UC-GCUCCA-CCGgGCCGa-----------GCGGCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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