Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23513 | 5' | -58.5 | NC_005259.1 | + | 19078 | 0.7 | 0.430715 |
Target: 5'- gCgGUCCGGCGCUacgacaaGCUGCG-CGAa -3' miRNA: 3'- -GgCAGGCCGCGAguag---UGGCGCuGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 18326 | 0.68 | 0.516638 |
Target: 5'- aCCGaggCCGGUGCguUCGUggucgaCGCCGaCGGCGAc -3' miRNA: 3'- -GGCa--GGCCGCG--AGUA------GUGGC-GCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 17352 | 0.68 | 0.534677 |
Target: 5'- gCCGUCgaggucggcggcuCGGCGCUgGUCguggucgACUGCGACa- -3' miRNA: 3'- -GGCAG-------------GCCGCGAgUAG-------UGGCGCUGcu -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 17022 | 0.66 | 0.629468 |
Target: 5'- aCCGccaUCCucGGCGCUggCAUCgacaACCGCGACc- -3' miRNA: 3'- -GGC---AGG--CCGCGA--GUAG----UGGCGCUGcu -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 14723 | 0.67 | 0.56726 |
Target: 5'- aCG-CC-GCGCUCGUCGCCGaGAUGu -3' miRNA: 3'- gGCaGGcCGCGAGUAGUGGCgCUGCu -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 14039 | 0.66 | 0.639889 |
Target: 5'- aUCGagCGGCaGCgCAUCGuCgGCGACGAg -3' miRNA: 3'- -GGCagGCCG-CGaGUAGU-GgCGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 13454 | 0.72 | 0.307393 |
Target: 5'- aCCGUCggcgCGGCGCUgGugcUCACCGCgcugcccGACGAu -3' miRNA: 3'- -GGCAG----GCCGCGAgU---AGUGGCG-------CUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 13045 | 0.66 | 0.639889 |
Target: 5'- aCGUUCGGCcCgu-UCugCGCGAUGGu -3' miRNA: 3'- gGCAGGCCGcGaguAGugGCGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 9503 | 0.68 | 0.536696 |
Target: 5'- -aGgaaCGuGCGCUCGUCGCgCGCGuCGAc -3' miRNA: 3'- ggCag-GC-CGCGAGUAGUG-GCGCuGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 7404 | 0.71 | 0.38674 |
Target: 5'- cCCGcaaCGGCaucCUCGUCGCCGuCGGCGGu -3' miRNA: 3'- -GGCag-GCCGc--GAGUAGUGGC-GCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 4331 | 0.69 | 0.487142 |
Target: 5'- cUCGUgCCGGaC-CUCGUCGCgGUGAUGAg -3' miRNA: 3'- -GGCA-GGCC-GcGAGUAGUGgCGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 752 | 0.71 | 0.353758 |
Target: 5'- gCCG-CCGGUGCcuaccgCGUcCACCGCGcCGAg -3' miRNA: 3'- -GGCaGGCCGCGa-----GUA-GUGGCGCuGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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